Overview

  • Product name
    Anti-IL22 Receptor Alpha antibody
    See all IL22 Receptor Alpha primary antibodies
  • Description
    Rabbit polyclonal to IL22 Receptor Alpha
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    QSSNFENILTWDSGPE

    , corresponding to amino acids 31-46 of Human IL-22 Receptor.

  • Positive control
    • WB: HepG2 whole cell lysate. IHC-P: Rat kidney tissue. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab5984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.Can be blocked with IL22 Receptor Alpha peptide (ab6229).
IHC-P Use a concentration of 5 µg/ml. PubMed: 19535621
ICC Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Anti-IL22 Receptor Alpha antibody images

  • Lane 1 : Anti-IL22 Receptor Alpha antibody (ab5984) at 0.5 µg/ml
    Lane 2 : Anti-IL22 Receptor Alpha antibody (ab5984) at 1 µg/ml

    Lane 1 : HepG2 cell lysates
    Lane 2 : HepG2 cell lysates
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling IL22 Receptor Alpha with ab5984 at 5µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling IL22 Receptor Alpha with ab5984 at 20µg/ml.

  • Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling IL22 Receptor Alpha with ab5984 at 10µg/ml.

  • Immunocytochemistry of IL-22R in HepG2 cells with IL-22R antibody at 10 µg/ml.
  • Western blot analysis of IL-22R expression in human HepG2 cell lysate with ab5984 at 1µg /ml. Western blot analysis of IL-22R expression in human HepG2 cell lysate with ab5984 at 1µg /ml.

References for Anti-IL22 Receptor Alpha antibody (ab5984)

This product has been referenced in:
  • Ikeda S  et al. Disruption of CCL20-CCR6 interaction inhibits metastasis of advanced cutaneous T-cell lymphoma. Oncotarget 7:13563-74 (2016). WB . Read more (PubMed: 26789110) »
  • Andoh A  et al. Expression of IL-24, an activator of the JAK1/STAT3/SOCS3 cascade, is enhanced in inflammatory bowel disease. J Immunol 183:687-95 (2009). IHC-P ; Human . Read more (PubMed: 19535621) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris NuPAGE)
Loading amount
40 µg
Specification
Lung
Blocking step
Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Pete Serbedzija

Verified customer

Submitted Jul 05 2016

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (Lung)
Specification
Lung
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Mar 25 2014

Application
Flow Cytometry
Fixation
Surface staining
Permeabilization
No
Sample
Human Cell (Epidermal keratinocytes)
Specification
Epidermal keratinocytes
Gating Strategy
Human epidermal keratinocytes
Preparation
Cell harvesting/tissue preparation method: Trypsinization
Sample buffer: Biolegend cell staining buffer
Username

Abcam user community

Verified customer

Submitted Feb 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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