Overview

  • Product nameAnti-IL23 antibody
    See all IL23 primary antibodies
  • Description
    Rabbit polyclonal to IL23
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 16 amino acid synthetic peptide conjugated to KLH, corresponding to the C-terminus of Human IL23.

  • Positive control
    • Human Kidney, Mouse brain, Mouse kidney and Mouse liver tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab115759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.

Target

  • FunctionAssociates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Tissue specificitySecreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Developmental stageExpressed by newborns dendritic cells.
  • Cellular localizationSecreted. Secreted upon association with IL12B.
  • Information by UniProt
  • Database links
  • Alternative names
    • IL 23 A antibody
    • IL 23 antibody
    • IL 23 subunit alpha antibody
    • IL 23A antibody
    • IL 23p19 antibody
    • IL-23 subunit alpha antibody
    • IL-23-A antibody
    • IL-23p19 antibody
    • IL12B antibody
    • IL23 antibody
    • Il23a antibody
    • IL23A_HUMAN antibody
    • IL23P19 antibody
    • interleukin 12B antibody
    • Interleukin 23 alpha subunit p19 antibody
    • Interleukin 23 p19 subunit antibody
    • interleukin 23 subunit alpha antibody
    • interleukin 23 subunit p19 antibody
    • interleukin six, G CSF related factor antibody
    • Interleukin-23 subunit alpha antibody
    • Interleukin-23 subunit p19 antibody
    • JKA3 induced upon T cell activation antibody
    • MGC79388 antibody
    • P19 antibody
    • SGRF antibody
    see all

Anti-IL23 antibody images

  • All lanes : Anti-IL23 antibody (ab115759) at 1 µg/ml

    Lane 1 : Raji cell lysate
    Lane 2 : Raji cell lysate


    Predicted band size : 21 kDa

    Western blot of IL-23 in Raji cell lysate with ab115759 at 1 ug/ml in the (1) absence and (2) presence of blocking peptide.

  • Human Kidney (formalin-fixed, paraffin-embedded) stained with ab115759 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Mouse Brain (formalin-fixed, paraffin-embedded) stained with ab115759 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Mouse Kidney (formalin-fixed, paraffin-embedded) stained with ab115759 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Mouse Liver (formalin-fixed, paraffin-embedded) stained with ab115759 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-IL23 antibody (ab115759)

This product has been referenced in:
  • Szkandera J  et al. A functional germline variant in GLI1 implicates hedgehog signaling in clinical outcome of stage II and III colon carcinoma patients. Clin Cancer Res 20:1687-97 (2014). Human . Read more (PubMed: 24470513) »
  • Helbling M  et al. Investigation of IL-23 (p19, p40) and IL-23R identifies nuclear expression of IL-23 p19 as a favorable prognostic factor in colorectal cancer: a retrospective multicenter study of 675 patients. Oncotarget 5:4671-82 (2014). IHC . Read more (PubMed: 25015728) »

See all 2 Publications for this product

Product Wall

Vielen Dank für Ihre Anfrage.

Ich kann Ihnen ab115759 (Anti-IL23 antibody) empfehlen.

http://www.abcam.com/index.html?datasheet=115759 (or use the following: http://www.abcam.com/index.html?datasheet=115759).

Dieser A...

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Thank you for contacting us.

The bovine protein is 81% similar to human protein so the possibility of cross reactivity is high. Unfortunately for monoclonal antibodies we do not know the exact epitope sequence so I am sorry I can't be more spe...

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Thank you for contacting us.

The C-terminal sequence of IL23A protein is quite homologous between Bovina and Human. http://www.uniprot.org/blast/uniprot/2012102343Z34R5CGA?alignment=25

It seems that the antibody e.g. ab115759; http://...

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Vielen Dank für Ihren Anruf.

Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

/Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Frageboge...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"