Recombinant
RabMAb

Anti-IL23 antibody [EPR5585(N)] - C-terminal (ab190356)

Overview

  • Product name
    Anti-IL23 antibody [EPR5585(N)] - C-terminal
    See all IL23 primary antibodies
  • Description
    Rabbit monoclonal [EPR5585(N)] to IL23 - C-terminal
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human IL23 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NPF7

  • Positive control
    • THP-1 treated with GM-CSF+IL-4, THP-1 and Raji cell lysates; IL23 recombinant protein (Human IL23 p40 (aa23 -aa328) & Human IL23 P19 ( aa21-aa189) (65KDa)).
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR5585(N)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Function
    Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Tissue specificity
    Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Developmental stage
    Expressed by newborns dendritic cells.
  • Cellular localization
    Secreted. Secreted upon association with IL12B.
  • Information by UniProt
  • Database links
  • Alternative names
    • IL 23 A antibody
    • IL 23 antibody
    • IL 23 subunit alpha antibody
    • IL 23A antibody
    • IL 23p19 antibody
    • IL-23 subunit alpha antibody
    • IL-23-A antibody
    • IL-23p19 antibody
    • IL12B antibody
    • IL23 antibody
    • Il23a antibody
    • IL23A_HUMAN antibody
    • IL23P19 antibody
    • interleukin 12B antibody
    • Interleukin 23 alpha subunit p19 antibody
    • Interleukin 23 p19 subunit antibody
    • interleukin 23 subunit alpha antibody
    • interleukin 23 subunit p19 antibody
    • interleukin six, G CSF related factor antibody
    • Interleukin-23 subunit alpha antibody
    • Interleukin-23 subunit p19 antibody
    • JKA3 induced upon T cell activation antibody
    • MGC79388 antibody
    • P19 antibody
    • SGRF antibody
    see all

Images

  • All lanes : Anti-IL23 antibody [EPR5585(N)] - C-terminal (ab190356) at 1/1000 dilution

    Lane 1 : THP-1 treated with GM-CSF+IL-4 cell lysate
    Lane 2 : Untreated THP-1 cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
  • Anti-IL23 antibody [EPR5585(N)] - C-terminal (ab190356) at 1/200 dilution + IL23 recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 21 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References

ab190356 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190356.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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