Overview

  • Product name
  • Description
    Rabbit polyclonal to IL24
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide corresponding to a region within amino acids 100-150 of Human IL24

  • Positive control
    • Human prostate tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab115207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. After incubation with the primary antibody, slides are incubated with biotinylated rabbit anti-goat IgG secondary antibody followed by alkaline phosphatase-streptavidin and chromogen.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation.
  • Tissue specificity
    Up-regulated in melanoma cells induced to terminally differentiate.
  • Sequence similarities
    Belongs to the IL-10 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C49A antibody
    • FISP antibody
    • IL 24 antibody
    • IL 4 induced secreted protein antibody
    • IL-24 antibody
    • IL10B antibody
    • Il24 antibody
    • IL24_HUMAN antibody
    • interleukin 24 antibody
    • Interleukin-24 antibody
    • MDA-7 antibody
    • MDA7 antibody
    • Melanocyte associated Mda 7 antibody
    • Melanoma differentiation association protein 7 antibody
    • Melanoma differentiation-associated gene 7 protein antibody
    • Mob5 antibody
    • ST16 antibody
    • Suppression of tumorigenicity 16 (melanoma differentiation) antibody
    • Suppression of tumorigenicity 16 antibody
    • Suppression of tumorigenicity 16 protein antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue, staining IL24 with ab114207 at 10 µg/ml.
  • All lanes : Anti-IL24 antibody (ab115207) at 0.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with IL24 blocking peptide


    Predicted band size : 24 kDa
  • Fow cytometry analysis of IL24 in Jurkat cells using ab115207at 5 µg/106 cells. The shaded histogram represents cells only, green represents isotype control, and pink represents anti-IL24 antibody.

References

This product has been referenced in:
  • Margaryan NV  et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci 17:418 (2016). WB . Read more (PubMed: 27011171) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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