Overview

  • Product nameAnti-IL27 antibody
    See all IL27 primary antibodies
  • Description
    Rabbit polyclonal to IL27
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to 16 amino acids from near the N terminus of Human IL27.

  • Positive control
    • Human Lung tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 27 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.

Target

  • FunctionCytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation.
  • Tissue specificityExpressed in monocytes and in placenta.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationSecreted. Does not seem to be secreted without coexpression of EBI3.
  • Information by UniProt
  • Database links
  • Alternative names
    • EBI3 antibody
    • IL 27 antibody
    • IL 27 p28 subunit antibody
    • IL 27A antibody
    • IL 30 antibody
    • IL-27 subunit alpha antibody
    • IL-27-A antibody
    • IL27 antibody
    • IL27-A antibody
    • IL27A antibody
    • IL27A_HUMAN antibody
    • IL27p28 antibody
    • IL30 antibody
    • Interleukin 27 antibody
    • Interleukin 27 subunit alpha antibody
    • Interleukin 27, 28-KD subunit antibody
    • Interleukin 30 antibody
    • Interleukin-27 subunit alpha antibody
    • p28 antibody
    see all

Anti-IL27 antibody images

  • ab115671, at 5µg/ml, staining IL27 in Formalin-fixed, Paraffin-embedded Human Lung tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-IL27 antibody (ab115671)

This product has been referenced in:
  • Yin N  et al. IL-27 activates human trophoblasts to express IP-10 and IL-6: implications in the immunopathophysiology of preeclampsia. Mediators Inflamm 2014:926875 (2014). IHC-P . Read more (PubMed: 24659862) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Spleen)
Gel Running Conditions Reduced Denaturing (4-15%)
Loading amount 25 µg
Specification Spleen
Blocking step Milk as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 15 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cynomolgus Monkey Tissue sections (macaque retina)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
Permeabilization No
Specification macaque retina
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
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Submitted Mar 04 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Muller cell line MIO-M1)
Permeabilization Yes - 0.1 % TX-100
Specification Muller cell line MIO-M1
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Mar 02 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"