Overview

  • Product name
    Anti-IL28 Receptor alpha antibody
    See all IL28 Receptor alpha primary antibodies
  • Description
    Rabbit polyclonal to IL28 Receptor alpha
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72 - 121 (EECAGTKELL CSMMCLKKQD LYNKFKGRVR TVSPSSKSPW VESEYLDYLF) of IL28 Receptor alpha (NP_775087)

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa.Can be blocked with IL28 Receptor alpha peptide (ab195307). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    IL28RA is a class II cytokine receptor that interacts with IL10RB to bind IL28A, IL28B, and IL29, which are related to type I interferons. These ligands play a critical role in response to microbial challenge and activate the JAK/STAT signaling system. It heterodimers with IL10RB. There are 4 isoforms as result of alternative splicing. It is widely expressed. IL28RA belongs to the type II cytokine receptor family, and contains 1 fibronectin type III domain.
  • Cellular localization
    Membrane; Single-pass type I membrane protein
  • Database links
  • Alternative names
    • Class II cytokine receptor CRF2/12 antibody
    • CRF2-12 antibody
    • CRF2/12 antibody
    • Cytokine receptor class-II member 12 antibody
    • Cytokine receptor family 2 member 12 antibody
    • IFN lambda R1 antibody
    • IFN-lambda receptor 1 antibody
    • IFNLR antibody
    • IFNLR1 antibody
    • IL-28 receptor subunit alpha antibody
    • il-28r1 antibody
    • IL28R alpha antibody
    • IL28RA antibody
    • Interferon lambda receptor antibody
    • Interferon lambda receptor 1 antibody
    • interleukin 28 alpha receptor antibody
    • interleukin 28 receptor A antibody
    • Interleukin 28 receptor alpha chain antibody
    • interleukin 28 receptor, alpha (interferon, lambda receptor) antibody
    • Interleukin 28 receptor, alpha antibody
    • interleukin or cytokine receptor 2 antibody
    • Interleukin-28 receptor subunit alpha antibody
    • LICR2 antibody
    • Likely interleukin or cytokine receptor 2 antibody
    see all

Images

  • Anti-IL28 Receptor alpha antibody (ab83865) at 1 µg/ml (5% skim milk / PBS) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Tian J  et al. Blocking the PI3K/AKT pathway enhances mammalian reovirus replication by repressing IFN-stimulated genes. Front Microbiol 6:886 (2015). Read more (PubMed: 26388843) »
  • Reemann P  et al. Expression of class II cytokine genes in children's skin. Acta Derm Venereol 94:386-92 (2014). Read more (PubMed: 24284923) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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