The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 22 kDa. The detection limit for recombinant Human IL28A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Use a concentration of 0.5 - 2 µg/ml. ab109820 in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL28A.
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL28A (Biotin) (ab84239). ab109820 in conjunction with ab84239 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL28A.
FunctionCytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
Sequence similaritiesBelongs to the IL-28/IL-29 family.