Recombinant Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5328(2)] to IL-28A + IL-28B
- Suitable for: WB
- Reacts with: Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-IL-28A + IL-28B antibody [EPR5328(2)] -
Description
Rabbit monoclonal [EPR5328(2)] to IL-28A + IL-28B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide within Human IL-28B aa 150-250 (C terminal). The exact sequence is proprietary.
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Positive control
- Human IL-28A and IL-28B recombinant protein
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5328(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
Target
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Cellular localization
IL-28A: Secreted. IL-28B: Secreted.
Images
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All lanes : Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713) at 1/1000 dilution
Lane 1 : Human IL-28A recombinant protein (aa 27 to 200)
Lane 2 : House made His-tagged full length human IL-28B recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 22 kDaExposure time
Lane 1: 40 seconds
Lane 2: 3 second
Buffer and concentration: 5% NFDM /TBST
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Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713) at 1/5000 dilution + Human IL-28A (amino acids 27-200) recombinant protein
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 120 secondsBlocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBST
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab126713 has not yet been referenced specifically in any publications.