The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionReceptor for interleukin-2.
Involvement in diseaseGenetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
ab111849 at 0.25 µg/10<> cells detecting CD25 in Rat strain Wistar Con-A Activated Thymocytes by Flow Cytometry. 89.9% more of the cells were stained than in the control (Mouse IgG1, kappa).
References for Anti-IL2RA antibody [MRC OX-39] (ab111849)
This product has been referenced in:
Whiteland JL et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem43:313-20 (1995).
Read more (PubMed: 7868861) »
Paterson DJ et al. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol24:1281-90 (1987).
Read more (PubMed: 2828930) »