Overview

  • Product nameAnti-IL2RA antibody [PC61.5.3] (Biotin)
    See all IL2RA primary antibodies
  • Description
    Rat monoclonal [PC61.5.3] to IL2RA (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IHC-Fr, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse B6.1 CTL Cell line.

  • Positive control
    • Mouse (BALB/c) T cell Blasts (Con A activated).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: 99% PBS, 0.5% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab112176 is purified from ascitic fluid via Protein G Chromatography.
  • ClonalityMonoclonal
  • Clone numberPC61.5.3
  • MyelomaP3x63-Ag8.653
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use 0.1-0.2µg for 106 cells. ab18403-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionReceptor for interleukin-2.
  • Involvement in diseaseGenetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similaritiesContains 2 Sushi (CCP/SCR) domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD25 antibody
    • IDDM10 antibody
    • IL 2 RA antibody
    • IL 2 receptor subunit alpha antibody
    • IL 2R alpha chain antibody
    • IL 2R subunit alpha antibody
    • IL-2 receptor subunit alpha antibody
    • IL-2-RA antibody
    • IL-2R subunit alpha antibody
    • IL2 RA antibody
    • IL2 Receptor alpha antibody
    • IL2 receptor subunit alpha antibody
    • IL2-RA antibody
    • IL2R alpha chain antibody
    • IL2R antibody
    • IL2R subunit alpha antibody
    • IL2RA antibody
    • IL2RA_HUMAN antibody
    • Interleukin 2 receptor alpha chain antibody
    • interleukin 2 receptor subunit alpha antibody
    • Interleukin 2 receptor, alpha antibody
    • Interleukin-2 receptor subunit alpha antibody
    • Ly 43 antibody
    • Ly43 antibody
    • OTTHUMP00000019030 antibody
    • OTTHUMP00000019031 antibody
    • OTTHUMP00000019032 antibody
    • p55 antibody
    • p55 chain antibody
    • T Cell Growth Factor Receptor antibody
    • TAC antigen antibody
    • TCGFR antibody
    see all

Anti-IL2RA antibody [PC61.5.3] (Biotin) images

  • ab112176 at 0.1µg/106 cells staining IL2RA in Mouse T Cell Blasts (Con A Activated) cells (BALB/c strain) by Flow Cytometry.
    Percentage of cells stained above control (Biotin Rat IgG1): 99.4%.

References for Anti-IL2RA antibody [PC61.5.3] (Biotin) (ab112176)

This product has been referenced in:
  • Lowenthal JW  et al. High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61. J Immunol 135:3988-94 (1985). Read more (PubMed: 3934270) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"