The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIndirect ELISA: use at 1/2000 to 1/20000
Plate coating: use at 1/500
Western Blot: use at 1/1000 - 1/5000.
This antibody can be used in two-site immunoassay for the determination of human IL-3.
It recognizes a different epitope than 1F4 (ab9814).
We recommend using this antibody (ab9815) as the coating antibody.
Useful for the detection of human IL-3 by Western blot.
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.