Overview

  • Product name
  • Description
    Rabbit polyclonal to IL3
  • Tested applications
    Suitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mIL-3 (mouse Interleukin-3)

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Neutralising
  • Application notes
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-3 (0.5 ng/ml), a concentration of 0.15 - 0.2 µg/ml of this antibody is required.

    ELISA:To detect mIL-3 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-3.

    Western Blot:To detect mIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • Function
      Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
      This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
    • Tissue specificity
      Activated T-cells, mast cells, natural killer cells.
    • Sequence similarities
      Belongs to the IL-3 family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Colony stimulating factor multiple antibody
      • Hematopoietic growth factor antibody
      • IL 3 antibody
      • IL-3 antibody
      • IL3 antibody
      • IL3_HUMAN antibody
      • Interleukin 3 (colony stimulating factor, multiple) antibody
      • Interleukin 3 antibody
      • Interleukin-3 antibody
      • Mast cell growth factor antibody
      • MCGF antibody
      • MGC79398 antibody
      • MGC79399 antibody
      • Multi CSF antibody
      • Multilineage colony stimulating factor antibody
      • Multipotential colony stimulating factor antibody
      • Multipotential colony-stimulating factor antibody
      • OTTHUMP00000065963 antibody
      • P cell stimulating factor antibody
      • P-cell-stimulating factor antibody
      see all

    References

    ab9726 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab9726.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up