Anti-IL31 antibody (ab102750)

Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Anti-IL31 antibody (ab102750) at 1/100 dilution + Mouse spleen tissue lysates at 35 µg

    Predicted band size : 18 kDa
  • ab102750, at 1/50 dilution, staining IL31 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.
  • ab102750 staining IL31 in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then permeabilized using 0.1% saponin/PBS, blocked with 4% serum for 30 minutes at 25°C and then incubated with ab102750 at a 1/75 dilution for 14 hours at 4°C. The secondary used was an FITC conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

    Green : ab102750
    Red : pgp 9.5
    Yellow : merge

    See Abreview

References

ab102750 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
Yes - 0.1% Tween 20
Specification
Tonsil
Blocking step
BioGenex Power Block 10 mins then BioGenex NGS Protein Block 30 mins as blocking agent for 40 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 19 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Cell (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 12 2015


Das Labor hat mir mitgeteilt, dass der WB unter reduzierten und denaturierten Bedingungen durchgefuehrt wurde.

Das genaue Protocol ist wie folgt:

Western Blotting Protocol

A. Preparation of cell lysates

1. Coll...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (PBMCs)
Loading amount
500000 cells
Specification
PBMCs
Treatment
see below (Additional notes)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gels)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Specification
Skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
Yes - 0,1% Saponin / PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 13 2011

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Specification
Skin
Fixative
Acetone
Permeabilization
Yes - Saponin 0.1% PBS, 30 minutes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Ms. Bryony Stott

Verified customer

Submitted May 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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