Anti-IL31 antibody (ab102750)

Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Anti-IL31 antibody (ab102750) at 1/100 dilution + Mouse spleen tissue lysates at 35 µg

    Predicted band size: 18 kDa

  • ab102750, at 1/50 dilution, staining IL31 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.
  • ab102750 staining IL31 in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then permeabilized using 0.1% saponin/PBS, blocked with 4% serum for 30 minutes at 25°C and then incubated with ab102750 at a 1/75 dilution for 14 hours at 4°C. The secondary used was an FITC conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

    Green : ab102750
    Red : pgp 9.5
    Yellow : merge

    See Abreview

References

ab102750 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Das Labor hat mir mitgeteilt, dass der WB unter reduzierten und denaturierten Bedingungen durchgefuehrt wurde.

Das genaue Protocol ist wie folgt:

Western Blotting Protocol

A. Preparation of cell lysates

1. Coll...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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