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Synthetic peptide, corresponding to a 15 amino acid sequence near the C terminal of Human IL32 protein
Our Abpromise guarantee covers the use of ab37158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).Can be blocked with Human IL32 peptide (ab39778). All 4 isoforms of IL32 can be detected by this antibody. The larger precursor can be detected at approximately 26kDa. A smaller band can sometimes be observed at approximately 12.5kDa. This consists of multiple isoforms (this can appear smeared as they are slightly different molecular weights).|
|ICC/IF||Use a concentration of 20 µg/ml.|
|ELISA||Use at an assay dependent concentration. PubMed: 18544573|