Overview

  • Product nameAnti-IL32 antibody
    See all IL32 primary antibodies
  • Description
    Rabbit polyclonal to IL32
  • Specificityab37158 recognises all 4 isoforms of IL-32 (100% sequence alignment to each).
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to a 15 amino acid sequence near the C terminal of Human IL32 protein

  • Positive control
    • Human spleen lysate, mouse EL4 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab37158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).Can be blocked with Human IL32 peptide (ab39778). All 4 isoforms of IL32 can be detected by this antibody. The larger precursor can be detected at approximately 26kDa. A smaller band can sometimes be observed at approximately 12.5kDa. This consists of multiple isoforms (this can appear smeared as they are slightly different molecular weights).
ICC/IF Use a concentration of 20 µg/ml.
ELISA Use at an assay dependent concentration. PubMed: 18544573

Target

  • FunctionCytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.
  • Tissue specificitySelectively expressed in lymphocytes. Expression more prominent in immunce cells than in non-immune cells.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IL 32alpha antibody
    • IL 32beta antibody
    • IL 32delta antibody
    • IL 32gamma antibody
    • IL-32 antibody
    • IL32 antibody
    • IL32_HUMAN antibody
    • Interleukin 32 antibody
    • Interleukin 32 small antibody
    • Interleukin 32 theta antibody
    • Interleukin-32 antibody
    • interleukin-32 eta antibody
    • Natural killer cell transcript 4 antibody
    • Natural killer cells protein 4 antibody
    • NK 4 antibody
    • NK4 antibody
    • OTTHUMP00000236040 antibody
    • OTTHUMP00000236041 antibody
    • OTTHUMP00000236042 antibody
    • OTTHUMP00000236043 antibody
    • OTTHUMP00000236044 antibody
    • OTTHUMP00000236045 antibody
    • OTTHUMP00000236046 antibody
    • OTTHUMP00000236047 antibody
    • OTTHUMP00000236048 antibody
    • OTTHUMP00000236049 antibody
    • OTTHUMP00000236050 antibody
    • OTTHUMP00000236051 antibody
    • OTTHUMP00000236052 antibody
    • OTTHUMP00000236053 antibody
    • OTTHUMP00000236054 antibody
    • OTTHUMP00000241545 antibody
    • OTTHUMP00000241602 antibody
    • OTTHUMP00000241603 antibody
    • OTTHUMP00000241604 antibody
    • OTTHUMP00000241605 antibody
    • OTTHUMP00000241606 antibody
    • OTTHUMP00000241607 antibody
    • OTTHUMP00000241608 antibody
    • OTTHUMP00000241609 antibody
    • OTTHUMP00000241610 antibody
    • OTTHUMP00000241611 antibody
    • OTTHUMP00000241612 antibody
    • TAIF antibody
    • TAIFa antibody
    • TAIFb antibody
    • TAIFc antibody
    • TAIFd antibody
    • Tumor necrosis factor alpha-inducing factor antibody
    see all

Anti-IL32 antibody images

  • Lane 1 : Anti-IL32 antibody (ab37158) at 5 µg/ml
    Lane 2 : Anti-IL32 antibody (ab37158) at 10 µg/ml

    Lane 1 : Human spleen cell lysate
    Lane 2 : Human spleen cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 26 kDa
    Observed band size : 12,26 kDa (why is the actual band size different from the predicted?)
    Two isoforms of IL32 can be seen


  • Predicted band size : 26 kDa
    Westen blot data for human spleen cell lysates (left lanes) and mouse EL4 cell lysates (right lanes).
  • IHC-P of Human spleen tissue with ab37158 at 10 µg/ml
  • Immunofluorescence of IL-32 in Human Spleen cells using ab37158 at 20 ug/ml.

References for Anti-IL32 antibody (ab37158)

This product has been referenced in:
  • Cho JS  et al. Toll-like receptor 4-mediated expression of interleukin-32 via the c-Jun N-terminal kinase/protein kinase B/cyclic adenosine monophosphate response element binding protein pathway in chronic rhinosinusitis with nasal polyps. Int Forum Allergy Rhinol 6:1020-1028 (2016). Read more (PubMed: 27173130) »
  • Nold-Petry CA  et al. IL-32 promotes angiogenesis. J Immunol 192:589-602 (2014). Human . Read more (PubMed: 24337385) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry.

I can confirm that ab37158 was tested under both reducing and denaturing conditions. Here is the standard protocol for sample sample preparation.

Spleen tissue lysate was prepared by homogenization in mod...

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Thank you for contacting us.

I have attached the filled out Exel spreadsheet with all the requested information. To be fair, all the information asked could be answered with a look on the datasheet...

In addition, I had a look thr...

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Thank you for your enquiry. The immunogen for this antibody is conserved in isoforms A-D and the precursor of IL-32. Please let me know if you have any more questions.

Thank you for your enquiry. Regarding ab37158 (IL32 antibody), this product has been tested on EL4 cell lysate. This cell lysate is available from Abcam (ab7183 - Mouse lymphoma cell line). I hope this information helps. Please don't hesitate t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"