Recombinant Anti-IL-32 antibody [EPR5067] (ab169781)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5067] to IL-32
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-IL-32 antibody [EPR5067]
See all IL-32 primary antibodies -
Description
Rabbit monoclonal [EPR5067] to IL-32 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human lymph node and tonsil lysates; Human fetal thymus lysate; HT29 and SW480 lysates; HT29 treated with TNF alpha, SW480 treated with INF gamma and 293T treated with TNF alpha and IFN gamma lysates. WB: Human tonsil lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5067 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 27 kDa.
|
Notes |
---|
WB
1/1000 - 1/5000. Predicted molecular weight: 27 kDa. |
Target
-
Function
Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. -
Tissue specificity
Selectively expressed in lymphocytes. Expression more prominent in immunce cells than in non-immune cells. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 9235 Human
- Omim: 606001 Human
- SwissProt: P24001 Human
- Unigene: 943 Human
-
Alternative names
- IL 32alpha antibody
- IL 32beta antibody
- IL 32delta antibody
see all
Images
-
Anti-IL-32 antibody [EPR5067] (ab169781) at 1/1000 dilution + Human tonsil lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM /TBST.
Loading Control: GAPDHThis antibody could detect different isoforms of IL-32. -
All lanes : Anti-IL-32 antibody [EPR5067] (ab169781) at 1/1000 dilution
Lane 1 : Human lymph node lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human fetal thymus lysate
Lane 4 : HT29 treated with TNF alpha lysate
Lane 5 : HT29 lysate
Lane 6 : SW480 treated with INF gamma lysate
Lane 7 : SW480 lysate
Lane 8 : 293T treated with TNF alpha and IFN gamma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 27 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab169781 has been referenced in 1 publication.
- Dos Santos JC et al. Cytokines and microbicidal molecules regulated by IL-32 in THP-1-derived human macrophages infected with New World Leishmania species. PLoS Negl Trop Dis 11:e0005413 (2017). PubMed: 28241012