Overview

  • Product name
  • Description
    Rabbit polyclonal to IL33
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    The purified antibody has been heated to 56°C for 30 minutes. Endotoxin content is estimated to be <10 pg/ml by the LAL method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/20000 - 1/100000.
    WB: 1/500 - 1/2000. Predicted molecular weight: 31 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
      In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100).
    • Tissue specificity
      Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
    • Sequence similarities
      Belongs to the IL-1 family. Highly divergent.
    • Domain
      The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.
    • Post-translational
      modifications
      The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.
    • Cellular localization
      Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).
    • Information by UniProt
    • Database links
    • Alternative names
      • C9orf26 antibody
      • CHROMOSOME 9 OPEN READING FRAME 26 antibody
      • DKFZp586H0523 antibody
      • DVS27 antibody
      • DVS27 related protein antibody
      • IL 1F11 antibody
      • IL 33 antibody
      • IL-1F11 antibody
      • IL-33 antibody
      • IL1F11 antibody
      • IL33 antibody
      • IL33_HUMAN antibody
      • Interleukin 1 family member 11 antibody
      • Interleukin 33 antibody
      • INTERLEUKIN 33 NFHEV antibody
      • Interleukin 33 precursor antibody
      • Interleukin-1 family member 11 antibody
      • Interleukin-33 (109-270) antibody
      • Interleukin33 antibody
      • NF HEV antibody
      • NF-HEV antibody
      • NFEHEV antibody
      • NFHEV antibody
      • Nuclear factor for high endothelial venules antibody
      • Nuclear factor from high endothelial venules antibody
      • OTTHUMP00000021041 antibody
      • RP11 575C20.2 antibody
      see all

    Images

    • Anti-IL33 antibody (ab92822) + Recombinant human IL33 (100-145)

      Predicted band size : 31 kDa

    References

    ab92822 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92822.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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