Anti-IL33 antibody [Nessy-1] (ab54385)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab54385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 31 kDa.
IP 1/100.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionCytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
    In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100).
  • Tissue specificityExpressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
  • Sequence similaritiesBelongs to the IL-1 family. Highly divergent.
  • DomainThe homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.
  • Post-translational
    modifications
    The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.
  • Cellular localizationNucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).
  • Information by UniProt
  • Database links
  • Alternative names
    • C9orf26 antibody
    • CHROMOSOME 9 OPEN READING FRAME 26 antibody
    • DKFZp586H0523 antibody
    • DVS27 antibody
    • DVS27 related protein antibody
    • IL 1F11 antibody
    • IL 33 antibody
    • IL-1F11 antibody
    • IL-33 antibody
    • IL1F11 antibody
    • IL33 antibody
    • IL33_HUMAN antibody
    • Interleukin 1 family member 11 antibody
    • Interleukin 33 antibody
    • INTERLEUKIN 33 NFHEV antibody
    • Interleukin 33 precursor antibody
    • Interleukin-1 family member 11 antibody
    • Interleukin-33 (109-270) antibody
    • Interleukin33 antibody
    • NF HEV antibody
    • NF-HEV antibody
    • NFEHEV antibody
    • NFHEV antibody
    • Nuclear factor for high endothelial venules antibody
    • Nuclear factor from high endothelial venules antibody
    • OTTHUMP00000021041 antibody
    • RP11 575C20.2 antibody
    see all

Anti-IL33 antibody [Nessy-1] images

  • All lanes : Anti-IL33 antibody [Nessy-1] (ab54385) at 1/1000 dilution

    Lane 1 : Liver Lysate
    Lane 2 : Liver Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse HRP conjugated polyclonal antibody at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-IL33 antibody [Nessy-1] (ab54385)

ab54385 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (liver, spleen)
Specification liver, spleen
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Nov 10 2014

Thank you for your enquiry and interest.

These two antibodies are from the same clone but one (ab54385) has no BSA and Azide in the storage buffer. The both have been tested for ELISA however, due to specific publication or feedback from cust...

Read More

I am sorry, I also meant to add, the Nessy-1 refers to the name of the hybridoma cell line this particular antibody is produced from. We also have this same antibody (produced from the same clone) with biotin conjugation that may be of interest to you....

Read More

Thank you for contacting us and your interest in our products.

I am sorry but there was some confusion in regards to this product (ab54385). It seems thatthis productwas removed from the catalogue in error. This has now been rectified and the...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"