• Product nameAnti-IL33 antibody [Nessy-1] (Biotin)
    See all IL33 primary antibodies
  • Description
    Mouse monoclonal [Nessy-1] to IL33 (Biotin)
  • ConjugationBiotin
  • SpecificityRecognizes pro IL33 (precursor) and mature IL33.
  • Tested applicationsSuitable for: ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 112-270 of Human IL33



Our Abpromise guarantee covers the use of ab54384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IHC-Fr 1/100. See PubMed 20160089.


  • FunctionCytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
    In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100).
  • Tissue specificityExpressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
  • Sequence similaritiesBelongs to the IL-1 family. Highly divergent.
  • DomainThe homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.
  • Post-translational
    The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.
  • Cellular localizationNucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).
  • Information by UniProt
  • Database links
  • Alternative names
    • C9orf26 antibody
    • DKFZp586H0523 antibody
    • DVS27 antibody
    • DVS27 related protein antibody
    • IL 1F11 antibody
    • IL 33 antibody
    • IL-1F11 antibody
    • IL-33 antibody
    • IL1F11 antibody
    • IL33 antibody
    • IL33_HUMAN antibody
    • Interleukin 1 family member 11 antibody
    • Interleukin 33 antibody
    • INTERLEUKIN 33 NFHEV antibody
    • Interleukin 33 precursor antibody
    • Interleukin-1 family member 11 antibody
    • Interleukin-33 (109-270) antibody
    • Interleukin33 antibody
    • NF HEV antibody
    • NF-HEV antibody
    • NFEHEV antibody
    • NFHEV antibody
    • Nuclear factor for high endothelial venules antibody
    • Nuclear factor from high endothelial venules antibody
    • OTTHUMP00000021041 antibody
    • RP11 575C20.2 antibody
    see all

Anti-IL33 antibody [Nessy-1] (Biotin) images

  • Skin biopsy samples from patients with psoriasis and control subjects were immediately placed in Tissue Freezing Medium and stored at-80 °C. Five sections (5 µm) were cut using a cryostat and fixed with cold acetone for 10 minutes. Immunohistochemical staining was performed using a LSAB+ system kit. Incubation with ab54384 at 1/100 dilution was performed for 30 minutes; secondary antibody was provided in the kit and was also used for 30 minutes, followed by appropriate washes. As negative control, the primary antibody was omitted.

References for Anti-IL33 antibody [Nessy-1] (Biotin) (ab54384)

This product has been referenced in:
  • Theoharides TC  et al. IL-33 augments substance P-induced VEGF secretion from human mast cells and is increased in psoriatic skin. Proc Natl Acad Sci U S A 107:4448-53 (2010). ICC ; Human . Read more (PubMed: 20160089) »

See 1 Publication for this product

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Thank you for your enquiry and interest.

These two antibodies are from the same clone but one (ab54385) has no BSA and Azide in the storage buffer. The both have been tested for ELISA however, due to specific publication or feedback from cust...

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Thank you very much for your interest our AbTrial program.

To our knowledge, ab54384 has not been tested in IHC-P. However by participating in our AbTrial program you can now use our products in an untested application or species without fin...

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I am very pleased to hear you would like to accept our offer and test ab54384 in IHC-P. This code will give you: 1 free PRIMARY ANTIBODYbefore the expirati...

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Thank you for contacting Abcam. As this antibody has not been tested in IHC-P, it does qualify for a testing discount. Please find the details below. If there is anything else I can help you with, please let me know. DISCOUNT CODE: ****** Expiration da...

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Thanks for your reply. According to the PNAS journal article, the skin samples were placed in Tissue Freezing Medium and stored at -80C before being cryosectioned. We have not tested ab54384 in IHC on frozen sections in our own labs, however the...

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Thank you for your reply and for pointing this information out to us. You are correct, the authors of the paper performed IHC on frozen tissue sections (IHC-Fr) and not ICC on cultured cells. Based on this information, we would recommend the antibody f...

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Thank you for contacting us. Immunocytochemistry was added to the datasheet based on a reference which used it for this application (PubMed ID 20160089). To our knowledge, the antibody has not been used for IHC, so I am afraid we do not know ho...

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