Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Western blot - Anti-IL36 alpha antibody (ab116279)
All lanes : Anti-IL36 alpha antibody (ab116279) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue with Immunising peptide at 1 µg/ml Lane 4 : Brain (Human) Tissue Lysate - fetal normal tissue with Immunising peptide at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution Developed using the ECL technique
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116279 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
References for Anti-IL36 alpha antibody (ab116279)
has not yet been referenced specifically in any publications.
Publishing research using ab116279? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"