Anti-IL36 alpha/IL-1F6 antibody - C-terminal (ab180909)
Key features and details
- Rabbit polyclonal to IL36 alpha/IL-1F6 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL36 alpha/IL-1F6 antibody - C-terminal
See all IL36 alpha/IL-1F6 primary antibodies -
Description
Rabbit polyclonal to IL36 alpha/IL-1F6 - C-terminal -
Host species
Rabbit -
Specificity
ab180909 will not cross-react with IL36 beta or IL36 gamma. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IL36 alpha/IL-1F6 (C terminal). (11 amino acid peptide; NP_055255).
Database link: Q9UHA7 -
Positive control
- MCF7 cell lysate; Human brain tissue; mouse brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa). |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Tissue specificity
Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 27179 Human
- Omim: 605509 Human
- SwissProt: Q9UHA7 Human
- Unigene: 278910 Human
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Alternative names
- FIL1 antibody
- FIL1 epsilon antibody
- FIL1(EPSILON) antibody
see all
Images
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All lanes : Anti-IL36 alpha/IL-1F6 antibody - C-terminal (ab180909) at 1 µg/ml
Lane 1 : MCF7 cell lysate
Lane 2 : MCF7 cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 18 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling IL36 alpha / IL-1F6 with ab180909 at 20 µg/ml.
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling IL36 alpha / IL-1F6 with ab180909 at 5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab180909 has been referenced in 6 publications.
- Xie X et al. Interleukin-36a suppresses growth of non-small cell lung cancer in vitro by reducing angiogenesis. FEBS Open Bio 11:1353-1363 (2021). PubMed: 33713575
- Chen F et al. IL-36?s in the colorectal cancer: is interleukin 36 good or bad for the development of colorectal cancer? BMC Cancer 20:92 (2020). PubMed: 32013927
- Frew JW et al. A systematic review and critical evaluation of inflammatory cytokine associations in hidradenitis suppurativa. F1000Res 7:1930 (2018). PubMed: 30828428
- Frew JW et al. A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa. F1000Res 7:1923 (2018). PubMed: 31281635
- Hessam S et al. Interleukin-36 in hidradenitis suppurativa: evidence for a distinctive proinflammatory role and a key factor in the development of an inflammatory loop. Br J Dermatol 178:761-767 (2018). PubMed: 28975626
- Russell SE et al. IL-36a expression is elevated in ulcerative colitis and promotes colonic inflammation. Mucosal Immunol 9:1193-204 (2016). PubMed: 26813344