Overview

  • Product name
  • Description
    Rabbit polyclonal to IL36 beta
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human IL36 beta aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9NZH7

  • Positive control
    • This antibody gave a positive signal in Human Tonsil tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 19 kDa).

Target

  • Tissue specificity
    Expression at low levels in tonsil, bone marrow, heart, placenta, lung, testis and colon but not in other tissues or in any hematopoietic cell lines.
  • Sequence similarities
    Belongs to the IL-1 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310043N20Rik antibody
    • Family of interleukin 1 eta antibody
    • FIL1 (ETA) antibody
    • FIL1 antibody
    • FIL1 eta antibody
    • FIL1H antibody
    • FILI-(ETA) antibody
    • IL 1F8 (FIL1 eta antibody
    • IL 1F8 antibody
    • IL 1H2 antibody
    • IL-1 eta antibody
    • IL-1F8 antibody
    • IL-1H2 antibody
    • IL1 ETA antibody
    • IL1F8 (Canonical product IL 1F8a) antibody
    • IL1F8 antibody
    • IL1F8_HUMAN antibody
    • IL1H2 antibody
    • IL36B antibody
    • Interleukin 1 family, member 8 (eta) antibody
    • Interleukin 1 family, member 8 antibody
    • Interleukin 1 homolog 2 antibody
    • Interleukin 1 Superfamily e antibody
    • Interleukin 36 beta antibody
    • Interleukin-1 eta antibody
    • Interleukin-1 family member 8 antibody
    • Interleukin-1 homolog 2 antibody
    • MGC126880 antibody
    • MGC126882 antibody
    • OTTMUSP00000012797 antibody
    • RP23-176J12.1 antibody
    see all

Images

  • All lanes : Anti-IL36 beta antibody (ab116280) at 1 µg/ml (Milk blocking 3%)

    Lane 1 : Tonsil (Human) Tissue Lysate
    Lane 2 : Tonsil (Human) Tissue Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Secondary
    Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa (possible non-specific binding).

    Exposure time : 20 minutes

    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab116280 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab116280 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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