Anti-IL36 beta/IL-1F8 antibody - C-terminal (ab180890)
Key features and details
- Rabbit polyclonal to IL36 beta/IL-1F8 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL36 beta/IL-1F8 antibody - C-terminal -
Description
Rabbit polyclonal to IL36 beta/IL-1F8 - C-terminal -
Host species
Rabbit -
Specificity
ab180890 will only detect the longer isoform of IL36 beta / IL-1F8. ab180890 will not cross-react with IL36 alpha or IL36 gamma. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IL36 beta/IL-1F8 (C terminal). (16 amino acid peptide; NP_055253).
Database link: Q9NZH7 -
Positive control
- A549 cell lysate; Human lung tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 19 kDa).
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IHC-P |
Use a concentration of 2.5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 19 kDa). |
IHC-P
Use a concentration of 2.5 - 20 µg/ml. |
Target
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Tissue specificity
Expression at low levels in tonsil, bone marrow, heart, placenta, lung, testis and colon but not in other tissues or in any hematopoietic cell lines. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 27177 Human
- Omim: 605508 Human
- SwissProt: Q9NZH7 Human
- Unigene: 278909 Human
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Alternative names
- 2310043N20Rik antibody
- Family of interleukin 1 eta antibody
- FIL1 (ETA) antibody
see all
Images
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All lanes : Anti-IL36 beta/IL-1F8 antibody - C-terminal (ab180890) at 1 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : A549 cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 19 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling IL36 beta / IL-1F8 with ab180890 at 2.5 μg/ml.
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Immunohistochemical analysis of Human lung tissue labeling IL36 beta / IL-1F8 with ab180890 at 20 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab180890 has been referenced in 4 publications.
- Chen F et al. IL-36?s in the colorectal cancer: is interleukin 36 good or bad for the development of colorectal cancer? BMC Cancer 20:92 (2020). PubMed: 32013927
- Hessam S et al. Interleukin-36 in hidradenitis suppurativa: evidence for a distinctive proinflammatory role and a key factor in the development of an inflammatory loop. Br J Dermatol 178:761-767 (2018). PubMed: 28975626
- Frew JW et al. A systematic review and critical evaluation of inflammatory cytokine associations in hidradenitis suppurativa. F1000Res 7:1930 (2018). PubMed: 30828428
- Frew JW et al. A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa. F1000Res 7:1923 (2018). PubMed: 31281635