Overview

  • Product nameAnti-IL36 gamma antibody
    See all IL36 gamma primary antibodies
  • Description
    Rabbit polyclonal to IL36 gamma
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human IL36 gamma aa 100 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9NZH8

  • Positive control
    • This antibody gave a positive signal in Human Tonsil tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

Target

  • FunctionFunction as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response.
  • Tissue specificityHighly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin.
  • Sequence similaritiesBelongs to the IL-1 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IL 1 epsilon antibody
    • IL 1 related protein 2 antibody
    • IL 1(EPSILON) antibody
    • IL 1F9 antibody
    • IL 1H1 antibody
    • IL 1RP2 antibody
    • IL-1 epsilon antibody
    • IL-1-related protein 2 antibody
    • IL-1F9 antibody
    • IL-1H1 antibody
    • IL-1RP2 antibody
    • IL1E antibody
    • Il1f9 antibody
    • IL1F9_HUMAN antibody
    • IL1H1 antibody
    • IL1RP2 antibody
    • IL36G antibody
    • Interleukin 1 epsilon antibody
    • Interleukin 1 family member 9 antibody
    • Interleukin 1 homolog 1 antibody
    • Interleukin 1 related protein 2 antibody
    • Interleukin 36 gamma antibody
    • Interleukin-1 epsilon antibody
    • Interleukin-1 family member 9 antibody
    • Interleukin-1 homolog 1 antibody
    see all

Anti-IL36 gamma antibody images

  • Anti-IL36 gamma antibody (ab116281) at 1 µg/ml + Tonsil (Human) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 19 kDa
    Additional bands at : 90 kDa (possible non-specific binding).

    Exposure time : 8 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116281 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References for Anti-IL36 gamma antibody (ab116281)

ab116281 has not yet been referenced specifically in any publications.

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