Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783)
Key features and details
- Mouse monoclonal [OTI2F4] to IL36 gamma/IL-1F9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-IL36 gamma/IL-1F9 antibody [OTI2F4]
See all IL36 gamma/IL-1F9 primary antibodies -
Description
Mouse monoclonal [OTI2F4] to IL36 gamma/IL-1F9 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human IL36 gamma/IL-1F9 aa 1-200.
Database link: Q9NZH8 -
Positive control
- HEK293T cells transfected with pCMV6-ENTRY IL-36 gamma/IL-1F9 cDNA; Human colon adenocarcinoma and pancreas tissues; HeLa cells
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General notes
Dilute in PBS (pH7.3) before use.
The clone number has been updated from 2F4 to OTI2F4, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 29th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab156783 is purified from TCS by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI2F4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/4000. Predicted molecular weight: 19 kDa.
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IHC-P |
1/150.
Perform heat-induced epitope retrieval with 10mM citric buffer, pH 6.0 at 120oC for 3 minutes. |
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ICC/IF |
1/100.
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Notes |
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WB
1/4000. Predicted molecular weight: 19 kDa. |
IHC-P
1/150. Perform heat-induced epitope retrieval with 10mM citric buffer, pH 6.0 at 120oC for 3 minutes. |
ICC/IF
1/100. |
Target
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Function
Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response. -
Tissue specificity
Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 56300 Human
- Omim: 605542 Human
- SwissProt: Q9NZH8 Human
- Unigene: 211238 Human
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Alternative names
- IL 1 epsilon antibody
- IL 1 related protein 2 antibody
- IL 1(EPSILON) antibody
see all
Images
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All lanes : Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783) at 1/4000 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY IL36 gamma/IL-1F9 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 19 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC213525) -
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling IL36 gamma/IL-1F9 with ab156783 at 1/150 dilution.
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IL36 gamma/IL-1F9 with ab156783 at 1/150 dilution
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Immunofluorescent analysis of HeLa cells labeling IL36 gamma/IL-1F9 using ab156783 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab156783 has been referenced in 14 publications.
- Merleev A et al. Proprotein convertase subtilisin/kexin type 9 is a psoriasis-susceptibility locus that is negatively related to IL36G. JCI Insight 7:N/A (2022). PubMed: 35862195
- Takeichi T et al. Ceramide Analysis in Combination With Genetic Testing May Provide a Precise Diagnosis for Self-Healing Collodion Babies. J Lipid Res 63:100308 (2022). PubMed: 36332686
- Lossius AH et al. Early transcriptional changes after UVB treatment in atopic dermatitis include inverse regulation of IL-36γ and IL-37. Exp Dermatol 30:249-261 (2021). PubMed: 33067891
- Morelli M et al. Experimental Methods for the Immunological Characterization of Paradoxical Psoriasis Reactions Induced by TNF-α Biologics. Methods Mol Biol 2248:155-165 (2021). PubMed: 33185874
- Chen F et al. IL-36?s in the colorectal cancer: is interleukin 36 good or bad for the development of colorectal cancer? BMC Cancer 20:92 (2020). PubMed: 32013927