Overview

  • Product nameAnti-IL37 antibody
    See all IL37 primary antibodies
  • Description
    Mouse monoclonal to IL37
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-219 of Human IL1F7

Properties

Applications

Our Abpromise guarantee covers the use of ab57187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Target

  • FunctionSuppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. This function requires SMAD3. Suppresses, or reduces, proinflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation.
  • Tissue specificityIn general, low constitutive expression, if any, in healthy tissues; high expression in inflammatory counterparts, including in synovial tissues from individuals with active rheumatoid arthritis. Isoform A, isoform B and isoform C are expressed in testis, colon, placenta, lung and lymph node. Isoform D and isoform E were found only in testis and bone marrow. Whereas only isoform A is found in brain, only isoform B in kidney and only isoform C in heart.
  • Sequence similaritiesBelongs to the IL-1 family.
  • Post-translational
    modifications
    Proteolytically converted to the mature form by CASP1.
  • Cellular localizationCytoplasm > cytosol. Nucleus. Secreted. Stimulation with IL1B leads to colocalization with SMAD3 mostly in perinuclear regions. Only the CASP1-cleaved mature form translocates into the nucleus upon LPS stimulation.
  • Information by UniProt
  • Database links
  • Alternative names
    • FIL1 antibody
    • FIL1 zeta antibody
    • FIL1(ZETA) antibody
    • FIL1Z antibody
    • IL 1 zeta antibody
    • IL 1F7 antibody
    • IL 1F7b (IL 1H4, IL 1H, IL 1RP1) antibody
    • IL 1H4 antibody
    • IL 1RP1 antibody
    • IL 1X antibody
    • IL 1X protein antibody
    • IL-1 zeta antibody
    • IL-1F7 antibody
    • IL-1H antibody
    • IL-1H4 antibody
    • IL-1RP1 antibody
    • IL-1X antibody
    • IL-37 antibody
    • IL1F7 (canonical product IL 1F7b) antibody
    • IL1F7 antibody
    • IL1F7_HUMAN antibody
    • IL1H4 antibody
    • IL1RP1 antibody
    • Interleukin 1 family member 7 antibody
    • interleukin 1 family, member 7 (zeta) antibody
    • Interleukin 1 homolog 4 antibody
    • Interleukin 1 related protein antibody
    • Interleukin 1 superfamily z antibody
    • Interleukin 1 zeta antibody
    • Interleukin 37 antibody
    • Interleukin-1 family member 7 antibody
    • Interleukin-1 homolog 4 antibody
    • Interleukin-1 zeta antibody
    • Interleukin-1-related protein antibody
    • Interleukin-23 antibody
    see all

Anti-IL37 antibody images

  • IL1F7 antibody (ab57187) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
  • Western blot against tagged recombinant protein immunogen using ab57187 IL1F7 antibody at 1ug/ml. Predicted band size of immunogen is 50 kDa
  • Overlay histogram showing A431 cells stained with ab57187 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57187, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-IL37 antibody (ab57187)

This product has been referenced in:
  • Zhao JJ  et al. Interleukin-37 mediates the antitumor activity in hepatocellular carcinoma: role for CD57+ NK cells. Sci Rep 4:5177 (2014). IHC-P ; Human . Read more (PubMed: 24898887) »

See 1 Publication for this product

Product Wall

Thank you for keeping me updated on this situation. The band at ~50 kDa on our datasheet is the recombinant, tagged fragment of IL37 that was used as an immunogen to create ab57187, and I would not expect you to see a band at this molecular weight in e...

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Thank you for your reply and for sending the image and information. It's a little difficult to see the bands on the image- is there a lot of background or are the bands very clear? Are there any differences between the reduced and non-reduced l...

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Thank you for contacting us with your question. The ~45 kDa band that is shown on the datasheet is from a Western blot against the recombinant tagged immunogen, which has a predicted molecular weight of around 50 kDa (the tag adds weight). There...

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