Overview

  • Product name
    Anti-IL37 antibody - C-terminal
    See all IL37 primary antibodies
  • Description
    Rabbit polyclonal to IL37 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 154-218 of Human IL37.

  • Positive control
    • A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cells; Human breast cancer tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. This function requires SMAD3. Suppresses, or reduces, proinflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation.
  • Tissue specificity
    In general, low constitutive expression, if any, in healthy tissues; high expression in inflammatory counterparts, including in synovial tissues from individuals with active rheumatoid arthritis. Isoform A, isoform B and isoform C are expressed in testis, colon, placenta, lung and lymph node. Isoform D and isoform E were found only in testis and bone marrow. Whereas only isoform A is found in brain, only isoform B in kidney and only isoform C in heart.
  • Sequence similarities
    Belongs to the IL-1 family.
  • Post-translational
    modifications
    Proteolytically converted to the mature form by CASP1.
  • Cellular localization
    Cytoplasm > cytosol. Nucleus. Secreted. Stimulation with IL1B leads to colocalization with SMAD3 mostly in perinuclear regions. Only the CASP1-cleaved mature form translocates into the nucleus upon LPS stimulation.
  • Information by UniProt
  • Database links
  • Alternative names
    • FIL1 antibody
    • FIL1 zeta antibody
    • FIL1(ZETA) antibody
    • FIL1Z antibody
    • IL 1 zeta antibody
    • IL 1F7 antibody
    • IL 1F7b (IL 1H4, IL 1H, IL 1RP1) antibody
    • IL 1H4 antibody
    • IL 1RP1 antibody
    • IL 1X antibody
    • IL 1X protein antibody
    • IL-1 zeta antibody
    • IL-1F7 antibody
    • IL-1H antibody
    • IL-1H4 antibody
    • IL-1RP1 antibody
    • IL-1X antibody
    • IL-37 antibody
    • IL1F7 (canonical product IL 1F7b) antibody
    • IL1F7 antibody
    • IL1F7_HUMAN antibody
    • IL1H4 antibody
    • IL1RP1 antibody
    • Interleukin 1 family member 7 antibody
    • interleukin 1 family, member 7 (zeta) antibody
    • Interleukin 1 homolog 4 antibody
    • Interleukin 1 related protein antibody
    • Interleukin 1 superfamily z antibody
    • Interleukin 1 zeta antibody
    • Interleukin 37 antibody
    • Interleukin-1 family member 7 antibody
    • Interleukin-1 homolog 4 antibody
    • Interleukin-1 zeta antibody
    • Interleukin-1-related protein antibody
    • Interleukin-23 antibody
    see all

Images

  • Anti-IL37 antibody - C-terminal (ab153889) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 24 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling IL37 with ab153889 at 1/250 dilution.
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling IL37 with ab153889 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

References

This product has been referenced in:
  • Liu L  et al. Interleukin 37 limits monosodium urate crystal-induced innate immune responses in human and murine models of gout. Arthritis Res Ther 18:268 (2016). IHC ; Human . Read more (PubMed: 27863506) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153889.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up