The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 106 cells.
Use 0.5µg for 106 cells in 100µl or 100µl whole blood. This antibody has been tested using immunofluorescent staining with flow cytometric analysis.
ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionThis is a receptor for interleukin-3.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 5 subfamily.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
References for Anti-IL3RA antibody [6H6] (Biotin) (ab21560)
This product has been referenced in:
Sun Q et al. Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist. Blood87:83-92 (1996).
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