Overview

  • Product name
  • Description
    Rabbit polyclonal to IL3RA
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human IL3RA.

  • Positive control
    • This antibody gave a positive signal in HeLa whole cell lysate as well as both Human Spleen and Human Thymus tissue lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function
    This is a receptor for interleukin-3.
  • Sequence similarities
    Belongs to the type I cytokine receptor family. Type 5 subfamily.
  • Domain
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD123 antibody
    • CD123 antigen antibody
    • hIL 3Ra antibody
    • hIL-3Ra antibody
    • hIL3Ra antibody
    • IL 3 receptor alpha SP2 isoform antibody
    • IL 3R alpha antibody
    • IL-3 receptor subunit alpha antibody
    • IL-3R subunit alpha antibody
    • IL-3R-alpha antibody
    • IL-3RA antibody
    • IL3 Receptor alpha antibody
    • IL3R antibody
    • Il3ra antibody
    • IL3RA_HUMAN antibody
    • IL3RAX antibody
    • IL3RAY antibody
    • IL3RX antibody
    • IL3RY antibody
    • Interleukin 3 receptor alpha chain antibody
    • Interleukin 3 receptor, alpha (low affinity) antibody
    • Interleukin 3 receptor, alpha antibody
    • Interleukin-3 Receptor alpha antibody
    • Interleukin-3 receptor subunit alpha antibody
    • Interleukin3 receptor antibody
    • Interleukin3 receptor, Y-chromosomal antibody
    see all

Images

  • All lanes : Anti-IL3RA antibody (ab109707) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 155 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds

References

ab109707 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab109707.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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