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Rabbit polyclonal to IL3RB (phospho Y593)
ab53695 detects endogenous levels of IL3RB only when phosphorylated at tyrosine 593.
Predicted to work with:
Synthetic phosphopeptide derived from human IL3RB around the phosphorylation site of tyrosine 593 (G-P-Y
Human brain tissue and RAW264.7 cell extracts treated with G-CSF (25ng/ml, 15mins).
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab53695 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
1/500 - 1/1000. Predicted molecular weight: 97 kDa.
High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 2 fibronectin type-III domains.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Information by UniProt
/granulocyte-macrophage colony-stimulating factor receptor, beta antibody
Immunohistochemistry (Paraffin-embedded sections) - IL3RB (phospho Y593) antibody (ab53695)
ab53695 at 1/50 dilution staining IL3RB in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Western blot - IL3RB (phospho Y593) antibody (ab53695)
All lanes : Anti-IL3RB (phospho Y593) antibody (ab53695) Lane 1 : HepG2 cells treated with EGF (200ng/ml, 15 minutes) Lane 2 : HepG2 cells treated with EGF (200ng/ml, 15 minutes) with blocking phosphopeptide Predicted band size: 97 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"