Anti-IL3RB (phospho Y593) antibody (ab53695)

Overview

  • Product name
    Anti-IL3RB (phospho Y593) antibody
    See all IL3RB primary antibodies
  • Description
    Rabbit polyclonal to IL3RB (phospho Y593)
  • Specificity
    ab53695 detects endogenous levels of IL3RB only when phosphorylated at tyrosine 593.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human IL3RB around the phosphorylation site of tyrosine 593 (G-P-YP-L-G).

  • Positive control
    • Human brain tissue and RAW264.7 cell extracts treated with G-CSF (25ng/ml, 15mins).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab53695 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 97 kDa.

Target

  • Function
    High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
  • Sequence similarities
    Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 2 fibronectin type-III domains.
  • Domain
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • /granulocyte-macrophage colony-stimulating factor receptor, beta antibody
    • Beta-C antibody
    • CD131 antibody
    • CDw131 antibody
    • Colony stimulating factor 2 receptor beta antibody
    • Colony stimulating factor 2 receptor beta low affinity (granulocyte macrophage) antibody
    • Common beta chain antibody
    • Csf2rb antibody
    • Cytokine receptor common beta chain antibody
    • Cytokine receptor common beta chain precursor (CDw131 antigen) antibody
    • Cytokine receptor common subunit beta antibody
    • GM CSF/IL 3/IL 5 receptor common beta chain antibody
    • GM-CSF/IL-3/IL-5 receptor common beta subunit antibody
    • IL3RB antibody
    • IL3RB_HUMAN antibody
    • IL5RB antibody
    • Interleukin 3 receptor, beta antibody
    • Interleukin 3 receptor/granulocyte macrophage colony stimulating factor 3 receptor beta (high affinity) antibody
    • Interleukin 5 receptor, beta antibody
    • SMDP5 antibody
    • stimulating factor 3 receptor, beta (high affinity) antibody
    see all

Images

  • ab53695 at 1/50 dilution staining IL3RB in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-IL3RB (phospho Y593) antibody (ab53695)

    Lane 1 : HepG2 cells treated with EGF (200ng/ml, 15 minutes)
    Lane 2 : HepG2 cells treated with EGF (200ng/ml, 15 minutes) with blocking phosphopeptide


    Predicted band size : 97 kDa

References

ab53695 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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