Highly pure (>98%) recombinant rIL-4 (rat Interleukin-4)
FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPBS, pH 7.4, no preservative, sterile filtered
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22315633
Use a concentration of 0.5 µg/ml.
Use a concentration of 0.1 - 0.2 µg/ml.
FunctionParticipates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Involvement in diseaseGenetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Sequence similaritiesBelongs to the IL-4/IL-13 family.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody (ab9811)Image from Gui J et al., Evid Based Complement Alternat Med. 2012;2012:893023. Epub 2012 Jan 23. Fig 2.; doi:10.1155/2012/893023; 23 January 2012, Evidence-Based Complementary and Alternative Medicine, Volume 2012 (2012), Article ID 893023
Immunohistochemical analysis of rat endometrial tissue, staining IL4 with ab9811.
Antigen retrieval was by heat mediation in a citrate buffer (pH 6) followed by blocking with 1% BSA for 30 minutes. Sections were incubated with primary antibody (1/50) overnight at 4°C. An HRP-goat anti-rabbit IgG was used as the secondary antibody (1/200). Staining was detected using DAB.
References for Anti-IL4 antibody (ab9811)
This product has been referenced in:
Wu J et al. Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype. J Neuroinflammation14:36 (2017).
Read more (PubMed: 28196545) »
Cabrera-Pastor A et al. In vivo administration of extracellular cGMP normalizes TNF-a and membrane expression of AMPA receptors in hippocampus and spatial reference memory but not IL-1ß, NMDA receptors in membrane and working memory in hyperammonemic rats. Brain Behav Immun57:360-70 (2016).
Read more (PubMed: 27189036) »