Anti-IL-4 antibody (ab9811)
Key features and details
- Rabbit polyclonal to IL-4
- Suitable for: Sandwich ELISA, WB, ELISA, IHC-P
- Reacts with: Rat
- Isotype: unknown
Overview
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Product name
Anti-IL-4 antibody
See all IL-4 primary antibodies -
Description
Rabbit polyclonal to IL-4 -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant full length protein corresponding to Rat IL-4. Highly pure (>98%) recombinant rIL-4 (rat Interleukin-4)
Database link: P20096 -
Positive control
- WB: Recombinant rat IL4 protein (ab9812).
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General notes
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
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WB |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. PubMed: 22315633
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Notes |
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Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. |
WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 22315633 |
Target
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Function
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
Involvement in disease
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 287287 Rat
- SwissProt: P20096 Rat
- Unigene: 108255 Rat
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Alternative names
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
see all
Images
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To detect rat IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84278 is required. Using this antibody in conjunction with an anti-rat IL-4 (ab9811) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant rat IL-4.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (16)
ab9811 has been referenced in 16 publications.
- Zhao RJ et al. Acupuncture Inhibits Morphine Induced-Immune Suppress via Antioxidant System. Evid Based Complement Alternat Med 2022:7971801 (2022). PubMed: 36317105
- Espinel-Mesa DX et al. Immunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes from Lippia alba Essential Oils in an Experimental Model of Chronic Chagas Disease. Antioxidants (Basel) 10:N/A (2021). PubMed: 34829722
- Wei Y et al. Exposure to ozone impacted Th1/Th2 imbalance of CD4+ T cells and apoptosis of ASMCs underlying asthmatic progression by activating lncRNA PVT1-miR-15a-5p/miR-29c-3p signaling. Aging (Albany NY) 12:25229-25255 (2020). PubMed: 33223504
- Yang J et al. The influence of PM2.5 on lung injury and cytokines in mice. Exp Ther Med 18:2503-2511 (2019). PubMed: 31572502
- Bi CS et al. Calcitriol suppresses lipopolysaccharide-induced alveolar bone damage in rats by regulating T helper cell subset polarization. J Periodontal Res 54:612-623 (2019). PubMed: 31095745