Overview

  • Product nameAnti-IL4 antibody [BVD4-1D11]
    See all IL4 primary antibodies
  • Description
    Rat monoclonal [BVD4-1D11] to IL4
  • SpecificityRecognises natural and recombinant mouse IL4. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein (Mouse).

  • General notesStorage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
ELISA Use a concentration of 2 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 16.8 kDa.

Target

  • FunctionParticipates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
  • Involvement in diseaseGenetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similaritiesBelongs to the IL-4/IL-13 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell growth factor 1 antibody
    • B cell IgG differentiation factor antibody
    • B Cell Stimulatory Factor 1 antibody
    • B-cell stimulatory factor 1 antibody
    • BCGF 1 antibody
    • BCGF1 antibody
    • Binetrakin antibody
    • BSF-1 antibody
    • BSF1 antibody
    • IGG1 induction factor antibody
    • IL 4 antibody
    • IL-4 antibody
    • IL4 antibody
    • IL4_HUMAN antibody
    • Il4e12 antibody
    • Interleukin 4 antibody
    • Interleukin 4 variant 2 antibody
    • Interleukin 4, isoform 1 antibody
    • Interleukin-4 antibody
    • Lymphocyte stimulatory factor 1 antibody
    • MGC79402 antibody
    • Pitrakinra antibody
    see all

Anti-IL4 antibody [BVD4-1D11] images

  • Anti-IL4 antibody [BVD4-1D11] (ab11524) + mouse IL-4 recombinant protein

    Predicted band size : 16.8 kDa

References for Anti-IL4 antibody [BVD4-1D11] (ab11524)

This product has been referenced in:
  • Razali FN  et al. Tumor suppression effect of Solanum nigrum polysaccharide fraction on Breast cancer via immunomodulation. Int J Biol Macromol 92:185-193 (2016). Read more (PubMed: 27365117) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

In this particular case, since you would be using the product in an application and on a species which we guarantee I would not be able to provide a testing discount.

This product is covered by our A...

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Thank you for your new contact. I am sorry for my delayed response but was out of office yesterday.

The immunogen was a recombinant full length mouse protein. As far as we are aware, the epitope has not been mapped yet so we do not have this ...

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Thank you for your enquiry regarding ab11524 and for taking the time to provide some useful details of the experiments.

I have read through the protocol and would make the following suggestions:

1) Try to block the non-specific bind...

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Thank you very much for sending over the technical details.

Would it be possible to send me an image as well so that I can take a look at the signal?

I look forward to hearing from you soon.

Thank you for your enquiry regarding ab11524.

As the datasheet indicates the immunogne was a recombinant full length protein (Mouse). This antibody has been tested and characterized in different application such as Flow Cyt, IHC-Fr, ELISA, WB...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which gu...

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Thank you for contacting us. We have about 30 products related to IL4 proteins. Could you please specify for which product you would be interested in getting more information. Please click on the following link to explore all anti IL4 an...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (atherosclerotic and aneurysmal lesions)
Specification atherosclerotic and aneurysmal lesions
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Galina Sukhova

Verified customer

Submitted Mar 15 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"