- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-IL4 antibody [EPR1118Y]
See all IL4 primary antibodies -
DescriptionRabbit monoclonal [EPR1118Y] to IL4
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivityReacts with: Human
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Immunogen
A synthetic peptide corresponding to residues in human IL4
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Positive control
- Human tonsil lysate
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR1118Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab62351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/2000. Predicted molecular weight: 17 kDa. | |
| ICC/IF | 1/500. |
Target
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FunctionParticipates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
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Involvement in diseaseGenetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
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Sequence similaritiesBelongs to the IL-4/IL-13 family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3565 Human
- Omim: 147780 Human
- SwissProt: P05112 Human
- Unigene: 73917 Human
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Alternative names
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
see all
Images
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![Flow Cytometry - Anti-IL4 antibody [EPR1118Y] (ab62351)](http://a.abcam.com/ps/products/62/ab62351/Images/ab62351-275012-ab62351FC1.jpg)
Flow Cytometry - Anti-IL4 antibody [EPR1118Y] (ab62351)Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling IL4 with unpurified ab62351 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Immunocytochemistry/ Immunofluorescence - Anti-IL4 antibody [EPR1118Y] (ab62351)](http://a.abcam.com/ps/products/62/ab62351/Images/ab62351-262046-ab62351-Ramos-methanol-500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL4 antibody [EPR1118Y] (ab62351)Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) labelling IL4 with purified ab62351 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Western blot - Anti-IL4 antibody [EPR1118Y] (ab62351)](http://a.abcam.com/ps/products/62/ab62351/Images/WB IL4.jpg)
Western blot - Anti-IL4 antibody [EPR1118Y] (ab62351)Anti-IL4 antibody [EPR1118Y] (ab62351) at 1/2000 dilution + Human tonsil lysate at 10 µg
Secondary
goat anti rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 14 kDa (why is the actual band size different from the predicted?)
Protocols
References
ab62351 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"