Purification notesab106023 is an IgG fraction antibody purified from monospecific
antiserum by a multi-step process which includes delipidation, salt
fractionation and ion exchange chromatography followed by extensive
dialysis against the buffer stated. ab106023 has been heated to 56°C for 30 minutes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).
1/10000 - 1/50000.
FunctionParticipates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Involvement in diseaseGenetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Sequence similaritiesBelongs to the IL-4/IL-13 family.