Overview

  • Product nameAnti-IL4I1 antibody
    See all IL4I1 primary antibodies
  • Description
    Rabbit polyclonal to IL4I1
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human IL4I1.

    (Peptide available as ab19824.)

  • Positive control
    • This antibody gave a positive signal in western blot against human testis tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 19436310
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

Target

  • RelevanceIL-4-induced gene-1 (Il4i1 or Fig1) is expressed in primarily immune tissues and genetically maps to a region of susceptibility to autoimmune disease. The predicted Il4i1 protein (IL4I1) sequence is most similar to apoptosis-inducing protein and Apoxin I, which are both l-amino acid oxidases.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • FIG 1 antibody
    • Fig 1 protein antibody
    • FIG1 antibody
    • Fig1 protein antibody
    • HCG2043070 antibody
    • hFIG 1 antibody
    • hFIG1 antibody
    • IL4 induced protein 1 antibody
    • Interleukin 4 induced 1 antibody
    • Interleukin 4 induced protein 1 antibody
    • Interleukin four induced 1 antibody
    • L amino acid oxidase antibody
    • LAAO antibody
    • LAO antibody
    • Protein Fig 1 antibody
    • Protein Fig1 antibody
    see all

Anti-IL4I1 antibody images

  • ICC/IF image of ab18524 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab18524, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Anti-IL4I1 antibody (ab18524) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

References for Anti-IL4I1 antibody (ab18524)

This product has been referenced in:
  • Marquet J  et al. Dichotomy between factors inducing the immunosuppressive enzyme IL-4-induced gene 1 (IL4I1) in B lymphocytes and mononuclear phagocytes. Eur J Immunol 40:2557-68 (2010). Read more (PubMed: 20683900) »
  • Carbonnelle-Puscian A  et al. The novel immunosuppressive enzyme IL4I1 is expressed by neoplastic cells of several B-cell lymphomas and by tumor-associated macrophages. Leukemia 23:952-60 (2009). IHC-P ; Human . Read more (PubMed: 19436310) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (BMDM)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 20 µg
Specification BMDM
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 10 2016

Thank you for contacting us and sorry for the delay in getting back to you.

I have looked into the reason why the Anti-IL4I1 antibody (ab18524) has been unavailable for some time now. Unfortunately, we have been trying to produce new batches ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"