Anti-IL4R (phospho Y497) antibody (ab61099)

Overview

  • Product nameAnti-IL4R (phospho Y497) antibody
    See all IL4R primary antibodies
  • Description
    Rabbit polyclonal to IL4R (phospho Y497)
  • SpecificityDetects endogenous levels of IL4R only when phosphorylated at tyrosine 497 (corresponding to mouse tyrosine 500)
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized phosphopeptide derived from human IL4R around the phosphorylation site of tyrosine 497 (P-A-YP-R-S).

  • Positive control
    • 293 and HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from rabbit antiserum by affinity chromatography, using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionReceptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
    Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
  • Tissue specificityIsoform 1 and isoform 2 are highly expressed in activated T-cells.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.
  • DomainThe extracellular domain represents the IL4 binding protein (IL4BP).
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Post-translational
    modifications
    On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.
    The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD124 antibody
    • IL 4R alpha antibody
    • IL-4 receptor subunit alpha antibody
    • IL-4-binding protein antibody
    • IL-4R subunit alpha antibody
    • IL-4R-alpha antibody
    • IL-4RA antibody
    • IL4-BP antibody
    • Il4r antibody
    • IL4R nirs variant 1 antibody
    • IL4RA antibody
    • IL4RA_HUMAN antibody
    • Interleukin 4 receptor alpha chain antibody
    • Interleukin 4 receptor antibody
    • sIL4Ralpha/prot antibody
    • Soluble IL-4 receptor subunit alpha antibody
    • Soluble IL-4R-alpha antibody
    • Soluble interleukin-4 receptor subunit alpha antibody
    see all

Anti-IL4R (phospho Y497) antibody images

  • All lanes : Anti-IL4R (phospho Y497) antibody (ab61099) at 1/500 dilution

    Lane 1 : Extracts from 293 cells
    Lane 2 : Extracts from 293 cells with immunizing phosphopeptide


    Predicted band size : 89 kDa
    Observed band size : 89 kDa
  • ICC/IF image of ab61099 (at a 1/500 dilution) stained HeLa cells, minus immunising peptide (left image) and plus immunising peptide (right image).

References for Anti-IL4R (phospho Y497) antibody (ab61099)

ab61099 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"