MKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQL VFLLSEAHTCIPEN NGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGN L TVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTY LEP SLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQH G SSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISR TPE VTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTV LH QDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTK NQV SLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVD KSR WQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Amino acids1 to 232
Additional sequence informationA DNA sequence encoding the signal peptide and extracellular domain of human IL-4 R alpha (aa 1-232) was fused to the Fc region of human IgG1 (aa 90-330). Protein expressed in modified human 293 cells
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityThe ED50 of IL4R - Fc Chimera is typically 45-60 ng/ml as measured by its ability to neutralize IL-4 mediated proliferation of the human growth factor dependent TF-1 cell line.
% by SDS-PAGE.
ab83756 consists of 15-35% carbohydrate by weight.
ab83756 contains N-linked oligosaccharides and may have O-linked oligosaccharides.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
IL 4R alpha
IL-4 receptor subunit alpha
IL-4R subunit alpha
IL4R nirs variant 1
Interleukin 4 receptor
Interleukin 4 receptor alpha chain
Soluble IL-4 receptor subunit alpha
Soluble interleukin-4 receptor subunit alpha
FunctionReceptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
Tissue specificityIsoform 1 and isoform 2 are highly expressed in activated T-cells.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily. Contains 1 fibronectin type-III domain.
DomainThe extracellular domain represents the IL4 binding protein (IL4BP). The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation. Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Post-translational modificationsOn IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction. The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.
Lane 1: MW markers; Lane 2: IL4R - Fc Chimera; Lane 3: IL4R - Fc Chimera treated with PNGase F to remove potential N-linked glycans; Lane 4: IL4R - Fc Chimera treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
Functional Studies - IL4R protein (Fc Chimera) (ab83756)
Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins. The triangle indicates theoretical pI and MW of the protein.
References for IL4RA protein (Fc Chimera) (ab83756)
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