Overview

  • Product name
  • Description
    Rabbit polyclonal to IL5
  • Tested applications
    Suitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hIL-5 (human Interleukin-5)

Properties

Applications

Our Abpromise guarantee covers the use of ab9624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Neutralising
  • Application notes
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5 (0.45 ng/ml), a concentration of 0.05 - 0.09 µg/ml of this antibody is required.

    ELISA:To detect hIL-5 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-5.

    Western Blot:To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • Function
      Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
    • Sequence similarities
      Belongs to the IL-5 family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • B-cell differentiation factor I antibody
      • Colony stimulating factor antibody
      • EDF antibody
      • Eosinophil differentiation factor antibody
      • IL-5 antibody
      • IL5 antibody
      • IL5_HUMAN antibody
      • Interleukin 5 (colony stimulating factor, eosinophil) antibody
      • Interleukin 5 antibody
      • Interleukin-5 antibody
      • T Cell Replacing Factor antibody
      • T-cell replacing factor antibody
      • TRF antibody
      see all

    References for Anti-IL5 antibody (ab9624)

    ab9624 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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