The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesNeutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5 (0.45 ng/ml), a concentration of 0.05 - 0.09 µg/ml of this antibody is required.
ELISA:To detect hIL-5 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-5.
Western Blot:To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionFactor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.