• Product nameAnti-IL6 antibody [10E5]
    See all IL6 primary antibodies
  • Description
    Mouse monoclonal [10E5] to IL6
  • Tested applicationsSuitable for: ICC/IF, Dot Blot, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Recombinant full length protein (Rat)

  • Positive control
    • Rat peritoneal macrophages stimulated with LPS in flow cytometry.



Our Abpromise guarantee covers the use of ab25072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19775197
Dot Blot Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 24 kDa.
Flow Cyt Use 0.25µg for 105 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    N- and O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interleukin BSF 2 antibody
    • B cell differentiation factor antibody
    • B cell stimulatory factor 2 antibody
    • B-cell stimulatory factor 2 antibody
    • BSF 2 antibody
    • BSF-2 antibody
    • BSF2 antibody
    • CDF antibody
    • CTL differentiation factor antibody
    • Hepatocyte stimulatory factor antibody
    • HGF antibody
    • HSF antibody
    • Hybridoma growth factor antibody
    • Hybridoma growth factor Interferon beta-2 antibody
    • IFN-beta-2 antibody
    • IFNB2 antibody
    • IL 6 antibody
    • IL-6 antibody
    • IL6 antibody
    • IL6_HUMAN antibody
    • Interferon beta 2 antibody
    • Interferon beta-2 antibody
    • Interleukin 6 antibody
    • Interleukin 6 (interferon beta 2) antibody
    • Interleukin BSF 2 antibody
    • Interleukin-6 antibody
    see all

References for Anti-IL6 antibody [10E5] (ab25072)

This product has been referenced in:
  • Majkutewicz I  et al. Dimethyl fumarate attenuates intracerebroventricular streptozotocin-induced spatial memory impairment and hippocampal neurodegeneration in rats. Behav Brain Res 308:24-37 (2016). IF ; Rat . Read more (PubMed: 27083302) »
  • Walker PA  et al. Direct intrathecal implantation of mesenchymal stromal cells leads to enhanced neuroprotection via an NFkappaB-mediated increase in interleukin-6 production. Stem Cells Dev 19:867-76 (2010). ICC/IF ; Rat . Read more (PubMed: 19775197) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Rabbit Tissue lysate - whole (LIVER)
Specification LIVER
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 19 2013

Thank you again for your patience with this reply.

I heard back from the supplying lab that this antibody had not been tested in-house for WB with lysates. However, the antibody has been for reported with liver lysates. The PMID numbers for r...

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Thank you for your enquiry and for your patience.

I can confirm that this particular product is sourced externally. I have been in contact with them in order to obtain this information for you, and regrettably have not yet recieved a reply...

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Gracias por u respuesta.

Me alegro que hayáis conseguido optimizar los resultados de ab25072. Para mandarnos los resultados en conejo de éste y otros anticuerpos, seguid las instrucciones indicadas en el siguiente link:
Read More

La proteína IL6 puede presentar modificaciones postraduccionales como glicosilaciones, lo cual explicaría la banda a 30KDa.

Te envío algunas imágenes encontradas en internet que muestran blots de IL6 con ...

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Muchas gracias por mandarnos el cuestionario y la imagen del blot.

De la imagen puedo deducir que el anticuerpo funciona perfectamente (en el control positivo la banda a 24KDa se aprecia claramente), pero además de la proteína d...

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