Overview

  • Product nameAnti-IL6 antibody
    See all IL6 primary antibodies
  • Description
    Rabbit polyclonal to IL6
  • Specificityab6672 detects a band at 25 kDa in Human lung tissue lysate and Mouse spleen tissue lysate, however the signal in mouse tissue is significantly lower. It also binds strongly to a protein at ~55 kDa in Human lung tissue extracts, which we believe represents a glycosylated form of IL6. ab6672 also detects several bands in Human lung tissue lysate within the region of 30-40 kDa. These may represent heteromers of IL6. Please be aware that this product has low homology with the mouse and rat sequence of IL6 (Rat, 40%; Mouse 41%, UniProt blast) and we therefore cannot guarantee reactivity in these species.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, RIA, IP, IHC-Fr, Neutralising, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    recombinant human IL-6 produced in E.coli.

  • Positive control
  • General notes

    IL-6 synonyms: plasmacytoma growth factor (PCT-GF),interferon-a-2 (IFN-a2), monocyte derived human B cellgrowth factor, B cell stimulating factor (BSF-2),hepatocyte stimulating factor (HSF), and interleukinhybridoma/plasmacytoma-1 (IL-HP1).

Properties

Applications

Our Abpromise guarantee covers the use of ab6672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Can be blocked with IL6 peptide (ab133194).
ELISA 1/1000 - 1/5000.
IHC-P 1/400 - 1/800.
RIA 1/4000 - 1/8000.
IP 1/400 - 1/800.
IHC-Fr 1/400 - 1/800.
Neutralising 1/400.
ICC/IF 1/500. See Abreview.

Target

  • FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interleukin BSF 2 antibody
    • B cell differentiation factor antibody
    • B cell stimulatory factor 2 antibody
    • B-cell stimulatory factor 2 antibody
    • BSF 2 antibody
    • BSF-2 antibody
    • BSF2 antibody
    • CDF antibody
    • CTL differentiation factor antibody
    • Hepatocyte stimulatory factor antibody
    • HGF antibody
    • HSF antibody
    • Hybridoma growth factor antibody
    • Hybridoma growth factor Interferon beta-2 antibody
    • IFN-beta-2 antibody
    • IFNB2 antibody
    • IL 6 antibody
    • IL-6 antibody
    • IL6 antibody
    • IL6_HUMAN antibody
    • Interferon beta 2 antibody
    • Interferon beta-2 antibody
    • Interleukin 6 antibody
    • Interleukin 6 (interferon beta 2) antibody
    • Interleukin BSF 2 antibody
    • Interleukin-6 antibody
    see all

Anti-IL6 antibody images

  • All lanes : Anti-IL6 antibody (ab6672) at 1/500 dilution

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Human lung tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Mouse lung tissue lysate

    Lysates/proteins at 20 µg per lane.


    Performed under reducing conditions.

    Additional bands at : 25 kDa,55 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.

    Tissue lysates were denatured for 10-15 minutes at 90ºC. ab6672 was incubated overnight at 4ºC and the secondary antibody for 1 hour at RT.

    ab6672 detects a band at 25 kDa in Human lung tissue lysate and Mouse spleen tissue lysate, however the signal in mouse tissue is significantly lower. It also binds strongly to a protein at ~55 kDa in Human lung tissue extracts, which we believe represents a glycosylated form of IL6. ab6672 also detects several bands in Human lung tissue lysate within the region of 30-40 kDa. These may represent heteromers of IL6. 

  • IHC image of IL6 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6672, 1/400, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab6672 staining IL6 in human WBC by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with acetone and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in PBS; 0.1% TX10; 1% Goat serum) for 16 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.

    See Abreview

References for Anti-IL6 antibody (ab6672)

This product has been referenced in:
  • Liu Z  et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR? and activating NF-?B pathway in mice adipocytes. Sci Rep 6:21382 (2016). WB ; Mouse . Read more (PubMed: 26888669) »
  • Faustino-Rocha AI  et al. Effects of lifelong exercise training on mammary tumorigenesis induced by MNU in female Sprague-Dawley rats. Clin Exp Med N/A:N/A (2016). WB ; Rat . Read more (PubMed: 27094311) »

See all 116 Publications for this product

Product Wall

Thank you for your reply.

For ab6672, it is supplied as a whole antiserum, this means that it is not a purified antibody. The concentration range of 80-85mg/ml, represents the total protein concentration, the actual concentration of the IL6 a...

Read More
Application Immunocytochemistry
Sample Human Cell (pulp)
Permeabilization Yes - 4%paraform
Specification pulp
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Kyungjoo Seong

Verified customer

Submitted Dec 14 2016

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (LIVER)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification LIVER
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Vicky

Verified customer

Submitted Dec 06 2016

Abreviews
Application Western blot
Sample Human Tissue lysate - whole (Placenta)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Placenta
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Vicky

Verified customer

Submitted Nov 29 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (HNSCC Tissue)
Antigen retrieval step Heat mediated
Permeabilization No
Specification HNSCC Tissue
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. Biswanath Majumder

Verified customer

Submitted Jul 01 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Treatment 20 ug/ml poly(I:C)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 17 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Antigen retrieval step None
Sample Mouse Tissue sections (cartilage and meniscus)
Specification cartilage and meniscus
Permeabilization No
Fixative zinc buffered formalin
Username

Abcam user community

Verified customer

Submitted Oct 29 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Antigen retrieval step Other
Sample Mouse Tissue sections (Free-floating Brain sections (6-OHDA lesioned))
Specification Free-floating Brain sections (6-OHDA lesioned)
Permeabilization Yes - 0.4% TritonX-100
Fixative Paraformaldehyde
Username

Dr. Venissa Machado

Verified customer

Submitted Oct 27 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Thermo Scientific Starting Block T20 Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100%
Antigen retrieval step None
Sample Human Tissue sections (esophagus)
Specification esophagus
Permeabilization No
Fixative Formaldehyde
Username

Tatiana Karakasheva

Verified customer

Submitted Oct 24 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Mouse Tissue sections (Skin)
Specification Skin
Permeabilization Yes - 0,3% Triton X
Fixative Perfusion fixed with paraformaldehyde and picric acid
Username

Abcam user community

Verified customer

Submitted Aug 04 2014

1-10 of 82 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"