Overview

  • Product nameAnti-IL6 antibody
    See all IL6 primary antibodies
  • Description
    Rabbit polyclonal to IL6
  • Specificityab6672 detects a band at 25 kDa in Human lung tissue lysate and Mouse spleen tissue lysate, however the signal in mouse tissue is significantly lower. It also binds strongly to a protein at ~55 kDa in Human lung tissue extracts, which we believe represents a glycosylated form of IL6. ab6672 also detects several bands in Human lung tissue lysate within the region of 30-40 kDa. These may represent heteromers of IL6. Please be aware that this product has low homology with the mouse and rat sequence of IL6 (Rat, 40%; Mouse 41%, UniProt blast) and we therefore cannot guarantee reactivity in these species.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, RIA, IP, IHC-Fr, Neutralising, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    recombinant human IL-6 produced in E.coli.

  • Positive control
  • General notes

    IL-6 synonyms: plasmacytoma growth factor (PCT-GF),interferon-a-2 (IFN-a2), monocyte derived human B cellgrowth factor, B cell stimulating factor (BSF-2),hepatocyte stimulating factor (HSF), and interleukinhybridoma/plasmacytoma-1 (IL-HP1).

Properties

Applications

Our Abpromise guarantee covers the use of ab6672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Can be blocked with IL6 peptide (ab133194).
ELISA 1/1000 - 1/5000.
IHC-P 1/400 - 1/800.
RIA 1/4000 - 1/8000.
IP 1/400 - 1/800.
IHC-Fr 1/400 - 1/800.
Neutralising 1/400.
ICC/IF 1/500. See Abreview.

Target

  • FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interleukin BSF 2 antibody
    • B cell differentiation factor antibody
    • B cell stimulatory factor 2 antibody
    • B-cell stimulatory factor 2 antibody
    • BSF 2 antibody
    • BSF-2 antibody
    • BSF2 antibody
    • CDF antibody
    • CTL differentiation factor antibody
    • Hepatocyte stimulatory factor antibody
    • HGF antibody
    • HSF antibody
    • Hybridoma growth factor antibody
    • Hybridoma growth factor Interferon beta-2 antibody
    • IFN-beta-2 antibody
    • IFNB2 antibody
    • IL 6 antibody
    • IL-6 antibody
    • IL6 antibody
    • IL6_HUMAN antibody
    • Interferon beta 2 antibody
    • Interferon beta-2 antibody
    • Interleukin 6 antibody
    • Interleukin 6 (interferon beta 2) antibody
    • Interleukin BSF 2 antibody
    • Interleukin-6 antibody
    see all

Anti-IL6 antibody images

  • All lanes : Anti-IL6 antibody (ab6672) at 1/500 dilution

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Human lung tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Mouse lung tissue lysate

    Lysates/proteins at 20 µg per lane.


    Performed under reducing conditions.

    Additional bands at : 25 kDa,55 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.

    Tissue lysates were denatured for 10-15 minutes at 90ºC. ab6672 was incubated overnight at 4ºC and the secondary antibody for 1 hour at RT.

    ab6672 detects a band at 25 kDa in Human lung tissue lysate and Mouse spleen tissue lysate, however the signal in mouse tissue is significantly lower. It also binds strongly to a protein at ~55 kDa in Human lung tissue extracts, which we believe represents a glycosylated form of IL6. ab6672 also detects several bands in Human lung tissue lysate within the region of 30-40 kDa. These may represent heteromers of IL6. 

  • This picture represents a tissue section from the wrist joint in collagen-induced arthritis in mice stained with ab6672.  This image was kindly supplied as part of the review submitted by Elizabeth Chlipala.
  • ab6672 staining IL6 in serum starved PC3 cells treated with TRAP-6 (ab120801), by ICC/IF. Increase in expression of IL6 correlates with increased concentration of TRAP-6, as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120801 (TRAP-6) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6672 (1/500 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Anti-IL6 antibody (ab6672) at 1/1000 dilutionFigure. Immunoblot using Abcam's anti- IL6 antibody. This blot shows detection of an IL-6 26kDa tagged fusion protein (~300 ng, lane 1, green, 800 nm channel). Minimal reactivity is observed against the tag (data not shown). Protein was resolved on a 4-20% Tris- Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of a fluorescent conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature. Molecular weight markers are shown for size comparison (red, 700 nm channel). Other detection systems will yield similar results.
  • All lanes : Anti-IL6 antibody (ab6672) at 1/1000 dilution

    Lane 1 : Mouse serum
    Lane 2 : Mouse serum

    Lysates/proteins at 0.3 µg per lane.

    Secondary
    IRDye® 800CW goat anti-rabbit IgG (H+L) polyclonal at 1/15000 dilution

    Performed under reducing conditions.

    Observed band size : 17 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Francesco Elia Marino

    See Abreview

  • Anti-IL6 antibody (ab6672) at 1/500 dilution + Endotoxin-stimulated human peripheral blood mononuclear cells
  • The samples are formalin-fixed, formic acid decalcified rat knee joints with type II collagen induced arthritis. This image was kindly supplied as part of the review submitted by Elizabeth Chlipala (at University of Colorado, USA).
  • IHC image of IL6 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6672, 1/400, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab6672 staining IL6 in Mouse pancreatic neoplastic lesion tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with PBS + Triton (0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated Goat anti-mrabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained with DAPI.

    See Abreview

  • ICC/IF image of ab6672 stained THP1 cells. The cells were treated using phorbol 12-myristate 13-acetate (PMA) to induce differentiation and encourage cell adhesion to plate, Lipopolysaccharide (LPS) (100ng/ml for 6 hours) to increase expression of IL6, and Befreldin (300ng/ml for 3 hours) to inhibit secretion of IL6. Following treatment the cells were 100% Methanol fixed (5 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6672 at 1in500 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti-rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab6672 staining IL6 in human WBC by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with acetone and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in PBS; 0.1% TX10; 1% Goat serum) for 16 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.

    See Abreview

  • ab6672 staining IL6 (red)  in Mouse mesenchimal SC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Tween-20 and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in 3% BSA) for 1 hour. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1:500) was used as the secondary antibody. Blue (DAPI) - nuclei.

    See Abreview

References for Anti-IL6 antibody (ab6672)

This product has been referenced in:
  • Liu Z  et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR? and activating NF-?B pathway in mice adipocytes. Sci Rep 6:21382 (2016). WB ; Mouse . Read more (PubMed: 26888669) »
  • Faustino-Rocha AI  et al. Effects of lifelong exercise training on mammary tumorigenesis induced by MNU in female Sprague-Dawley rats. Clin Exp Med N/A:N/A (2016). WB ; Rat . Read more (PubMed: 27094311) »

See all 109 Publications for this product

Product Wall

Thank you for your reply.

For ab6672, it is supplied as a whole antiserum, this means that it is not a purified antibody. The concentration range of 80-85mg/ml, represents the total protein concentration, the actual concentration of the IL6 a...

Read More
Abreviews
Application Western blot
Sample Human Tissue lysate - whole (Placenta)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Placenta
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Vicky

Verified customer

Submitted Nov 29 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (HNSCC Tissue)
Antigen retrieval step Heat mediated
Permeabilization No
Specification HNSCC Tissue
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. Biswanath Majumder

Verified customer

Submitted Jul 01 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Treatment 20 ug/ml poly(I:C)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 17 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Antigen retrieval step None
Sample Mouse Tissue sections (cartilage and meniscus)
Specification cartilage and meniscus
Permeabilization No
Fixative zinc buffered formalin
Username

Abcam user community

Verified customer

Submitted Oct 29 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Antigen retrieval step Other
Sample Mouse Tissue sections (Free-floating Brain sections (6-OHDA lesioned))
Specification Free-floating Brain sections (6-OHDA lesioned)
Permeabilization Yes - 0.4% TritonX-100
Fixative Paraformaldehyde
Username

Dr. Venissa Machado

Verified customer

Submitted Oct 27 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Thermo Scientific Starting Block T20 Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100%
Antigen retrieval step None
Sample Human Tissue sections (esophagus)
Specification esophagus
Permeabilization No
Fixative Formaldehyde
Username

Ms. Tatiana Karakasheva

Verified customer

Submitted Oct 24 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Mouse Tissue sections (Skin)
Specification Skin
Permeabilization Yes - 0,3% Triton X
Fixative Perfusion fixed with paraformaldehyde and picric acid
Username

Abcam user community

Verified customer

Submitted Aug 04 2014

Abreviews
Application Western blot
Loading amount 0.3 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12)
Sample Mouse Serum (Mouse Serum)
Specification Mouse Serum
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Jun 28 2014

The immunogen is full length biologically active IL-6 produced in E.Coli


1-10 of 81 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"