Overview

  • Product nameAnti-IL6 antibody
    See all IL6 primary antibodies
  • Description
    Rabbit polyclonal to IL6
  • Tested applicationsSuitable for: WB, ELISA, Neutralising, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mIL6 (mouse Interleukin-6)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 - 2 µg/ml. Recommended concentration is based on 100 µl/well antibody solution. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse IL6.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mouse IL6 (3 pg/ml), a concentration of 0.24-0.36 µg/ml of ab9730 is required
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL6 (Biotin) (ab84270). Recommended concentration is based on 100 µl/well antibody solution. When used as capture antibody with recommended pair, this allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse IL6. Can be used as capture antibody when paired with ab84270.

Target

  • FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interleukin BSF 2 antibody
    • B cell differentiation factor antibody
    • B cell stimulatory factor 2 antibody
    • B-cell stimulatory factor 2 antibody
    • BSF 2 antibody
    • BSF-2 antibody
    • BSF2 antibody
    • CDF antibody
    • CTL differentiation factor antibody
    • Hepatocyte stimulatory factor antibody
    • HGF antibody
    • HSF antibody
    • Hybridoma growth factor antibody
    • Hybridoma growth factor Interferon beta-2 antibody
    • IFN-beta-2 antibody
    • IFNB2 antibody
    • IL 6 antibody
    • IL-6 antibody
    • IL6 antibody
    • IL6_HUMAN antibody
    • Interferon beta 2 antibody
    • Interferon beta-2 antibody
    • Interleukin 6 antibody
    • Interleukin 6 (interferon beta 2) antibody
    • Interleukin BSF 2 antibody
    • Interleukin-6 antibody
    see all

References for Anti-IL6 antibody (ab9730)

ab9730 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I am sorry for the confusion. IL6 antibody (ab9730) can indeed be applied by in vivo neutralisation. For further details please see the antibody datasheet. I have removed the enquiry stating that it cannot be applied in t...

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Thank you for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

Thank you very much for your patience and for the details that you have provided. I have been working with the antibody's originator to help determine what may be going on with your blot. The antibody was not tested against cell extracts/lysates (it wa...

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The murine IL-6 has a molecular weight of 21.7 kDa. On a western blot you would see a band at approx. 22 kDa.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"