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Our Abpromise guarantee covers the use of ab9730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|ELISA||Use a concentration of 0.5 - 2 µg/ml. Recommended concentration is based on 100 µl/well antibody solution. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse IL6.|
|Neutralising||Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mouse IL6 (3 pg/ml), a concentration of 0.24-0.36 µg/ml of ab9730 is required|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL6 (Biotin) (ab84270). Recommended concentration is based on 100 µl/well antibody solution. When used as capture antibody with recommended pair, this allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse IL6. Can be used as capture antibody when paired with ab84270.|
ab9730 has not yet been referenced specifically in any publications.