- Datasheet
- Specific References
- Protocols
Overview
- Product nameAnti-IL6 antibody (Biotin)
See all IL6 primary antibodies - DescriptionRabbit polyclonal to IL6 (Biotin)
- ConjugationBiotin
- Tested applicationsSuitable for: WB, ELISA, Sandwich ELISAmore details
- Species reactivityReacts with: Human
- Immunogen
Recombinant full length IL6 protein (Human) highly pure (>98%).
Properties
- FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... - Purification notesab84251 was purified by affinity chromatography and then biotinylated. Filter sterilised.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
- ELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab84251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 24 kDa. | |
| ELISA | Use a concentration of 0.25 - 1 µg/ml. Can be paired for ELISA with Mouse monoclonal to IL6 (ab9324). To detect hIL-6 by direct ELISA (using 100 µl/well antibody solution) a concentration of 0.25 – 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-6. | |
| Sandwich ELISA | Use at an assay dependent dilution. To detect hIL-6 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 – 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Mouse monoclonal to IL6 (ab9324) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-6. |
Target
- FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
- Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. - Sequence similaritiesBelongs to the IL-6 superfamily.
- Post-translational
modificationsN- and O-glycosylated. - Cellular localizationSecreted.
- Information by UniProt
-
Database links
- Entrez Gene: 3569 Human
- Omim: 147620 Human
- SwissProt: P05231 Human
- Unigene: 654458 Human
-
Alternative names
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
see all
References for Anti-IL6 antibody (Biotin) (ab84251)
ab84251 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"