IL6 overexpression 293T lysate (whole cell) (ab94059)

Overview

  • Product name
    IL6 overexpression 293T lysate (whole cell)
    See all IL6 lysates
  • Description
    IL6 overexpression lysate
  • General notes
    ab94059 is a 293T cell transfected lysate in which Human IL6 has been transiently over-expressed using a pCMV-IL6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Disease: Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. PTM: N- and O-glycosylated. Similarity: Belongs to the IL-6 superfamily.

Applications

Our Abpromise guarantee covers the use of ab94059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94059 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-IL6 antibody (ab66231) at 1/500 dilution

    Lane 1 : IL6 overexpression 293T lysate (whole cell) (ab94059) at 25 µg
    Lane 2 : 293T non transfected-lysate

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94059 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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