Overview

  • Product nameAnti-IL6R antibody [AP-MAB0848]
    See all IL6R primary antibodies
  • Description
    Rat monoclonal [AP-MAB0848] to IL6R
  • Tested applicationsSuitable for: IP, Flow Cyt, Inhibition Assay, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse recombinant IL-6R alpha chain

  • General notes


    This monoclonal can be used to block IL-6/IL-6R interactions.

Properties

Applications

Our Abpromise guarantee covers the use of ab83053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Inhibition Assay Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).

Target

  • FunctionPart of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
    Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
  • Tissue specificityIsoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • DomainThe two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • Post-translational
    modifications
    A short soluble form may also be released from the membrane by proteolysis.
  • Cellular localizationSecreted and Basolateral cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 126 antibody
    • CD126 antibody
    • CD126 antigen antibody
    • gp80 antibody
    • IL 6R 1 antibody
    • IL 6R alpha antibody
    • IL 6R antibody
    • IL-6 receptor alpha chain antibody
    • IL-6 receptor subunit alpha antibody
    • IL-6R 1 antibody
    • IL-6R subunit alpha antibody
    • IL-6R-alpha antibody
    • IL-6RA antibody
    • IL6Q antibody
    • Il6r antibody
    • IL6RA antibody
    • IL6RA_HUMAN antibody
    • IL6RQ antibody
    • Interleukin 6 receptor antibody
    • interleukin 6 receptor, alpha antibody
    • Interleukin-6 receptor subunit alpha antibody
    • Membrane glycoprotein 80 antibody
    • MGC30256 antibody
    see all

Anti-IL6R antibody [AP-MAB0848] images

  • ab83053 staining IL6R in Mouse stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 10% Milk for 150 minutes at 22°C. Samples were incubated with primary antibody (1/100 in 10% Milk) for 2.5 hours at 22°C. A Cy5®-conjugated Goat anti-rat IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

References for Anti-IL6R antibody [AP-MAB0848] (ab83053)

This product has been referenced in:
  • Mishra V  et al. Sex-specific IL-6-associated signaling activation in ozone-induced lung inflammation. Biol Sex Differ 7:16 (2016). Mouse . Read more (PubMed: 26949510) »

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam.

The antibody ab83053 can be used to prevent IL6 binding to its receptor and therefore could be used in a neutralizing type assay.

Please let me know if there is anything else I can help you with.

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (4–15%)
Sample Mouse Tissue lysate - whole (Lung)
Specification Lung
Blocking step BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 07 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (stem cells)
Specification stem cells
Fixative Methanol
Permeabilization No
Blocking step Milk as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Prof. U.R. Markert

Verified customer

Submitted Feb 08 2013

DISCOUNT CODE: xxxxxx
Ablaufdatum:26/03/2013


Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab83053 in ICC/IF zu testen, annehmen möchten. Wie besprochen, können Sie den Gutscheincode zum Kauf ei...

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Vielen Dank für Ihre Email.

Ich möchte mich noch einmal absichern, dass ich Sie richtig verstanden habe. Ist es richtig, dass Sie nach der Stimulation der Zellen eine ICC mit dem IL6R Antikörper durchführen wollen.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"