Overview

  • Product nameAnti-IL6R antibody [B-R6]
    See all IL6R primary antibodies
  • Description
    Mouse monoclonal [B-R6] to IL6R
  • SpecificityThis antibody recognizes soluble and membrane bound IL6R.
  • Tested applicationsSuitable for: IHC-Fr, Blocking, Inhibition Assay, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    IL6R (gp80) transfected CHO cell line.

  • Positive control
    • U266 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab47215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. The antibody blocks IL6 induced proliferation of XG-1 cells.
Inhibition Assay Use at an assay dependent concentration. The antibody inhibits IL6 binding to its receptor.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

Target

  • FunctionPart of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
    Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
  • Tissue specificityIsoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • DomainThe two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • Post-translational
    modifications
    A short soluble form may also be released from the membrane by proteolysis.
  • Cellular localizationSecreted and Basolateral cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 126 antibody
    • CD126 antibody
    • CD126 antigen antibody
    • gp80 antibody
    • IL 6R 1 antibody
    • IL 6R alpha antibody
    • IL 6R antibody
    • IL-6 receptor alpha chain antibody
    • IL-6 receptor subunit alpha antibody
    • IL-6R 1 antibody
    • IL-6R subunit alpha antibody
    • IL-6R-alpha antibody
    • IL-6RA antibody
    • IL6Q antibody
    • Il6r antibody
    • IL6RA antibody
    • IL6RA_HUMAN antibody
    • IL6RQ antibody
    • Interleukin 6 receptor antibody
    • interleukin 6 receptor, alpha antibody
    • Interleukin-6 receptor subunit alpha antibody
    • Membrane glycoprotein 80 antibody
    • MGC30256 antibody
    see all

Anti-IL6R antibody [B-R6] images

  • ab47215 staining IL6R on U266 cell line.

References for Anti-IL6R antibody [B-R6] (ab47215)

This product has been referenced in:
  • Touboul C  et al. Mesenchymal stem cells enhance ovarian cancer cell infiltration through IL6 secretion in an amniochorionic membrane based 3D model. J Transl Med 11:28 (2013). Human . Read more (PubMed: 23369187) »
  • Damman J  et al. Local renal complement C3 induction by donor brain death is associated with reduced renal allograft function after transplantation. Nephrol Dial Transplant 26:2345-54 (2011). Read more (PubMed: 21127132) »

See all 3 Publications for this product

Product Wall



I have added the protocol the lab uses to the end of this email for you. Please note that the experiment is done radioactively with Thymidine H3.

Regarding the amount of IL-6 found in the serum, 15pg/ml, it is 60 time less than the a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"