Overview

  • Product name
    Anti-IL6R antibody [B-R6] (FITC)
    See all IL6R primary antibodies
  • Description
    Mouse monoclonal [B-R6] to IL6R (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody recognises the Interleukin 6 Receptor alpha subunit (IL6 Ra, IL6R).
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human IL6R transfected with CHO cell line.

  • Positive control
    • Cells from U266 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab27321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
WB
  • Application notes
    Flow Cyt: Use Neat: 10µl for 106 cells or 100µl whole blood.
    IHC-Fr: Use at an assay dependent dilution (PMID 19658188).
    WB: Use at an assay dependent dilution (PMID 19658188).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
      Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
    • Tissue specificity
      Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
    • Sequence similarities
      Belongs to the type I cytokine receptor family. Type 3 subfamily.
      Contains 1 fibronectin type-III domain.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • Domain
      The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    • Post-translational
      modifications
      A short soluble form may also be released from the membrane by proteolysis.
    • Cellular localization
      Secreted and Basolateral cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 126 antibody
      • CD126 antibody
      • CD126 antigen antibody
      • gp80 antibody
      • IL 6R 1 antibody
      • IL 6R alpha antibody
      • IL 6R antibody
      • IL-6 receptor alpha chain antibody
      • IL-6 receptor subunit alpha antibody
      • IL-6R 1 antibody
      • IL-6R subunit alpha antibody
      • IL-6R-alpha antibody
      • IL-6RA antibody
      • IL6Q antibody
      • Il6r antibody
      • IL6RA antibody
      • IL6RA_HUMAN antibody
      • IL6RQ antibody
      • Interleukin 6 receptor antibody
      • interleukin 6 receptor, alpha antibody
      • Interleukin-6 receptor subunit alpha antibody
      • Membrane glycoprotein 80 antibody
      • MGC30256 antibody
      see all

    Images

    • ab27321 at 10µl/106 cells staining IL6R in U266 cell line by Flow Cytometry.

    References

    ab27321 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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