The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use Neat: 10µl for 106 cells or 100µl whole blood.
IHC-Fr: Use at an assay dependent dilution (PMID 19658188).
WB: Use at an assay dependent dilution (PMID 19658188).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPart of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
Tissue specificityIsoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily. Contains 1 fibronectin type-III domain. Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
DomainThe two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain. The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
Post-translational modificationsA short soluble form may also be released from the membrane by proteolysis.
Cellular localizationSecreted and Basolateral cell membrane.