The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at a concentration of 1 - 2 µg/ml.
Neut: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.
The exact concentration of antibody required to neutralize recombinant human IL7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity. The detection limit in immunoblotting for recombinant human IL7 is approximately 25 ng/lane and 300 ng/lane under nonreducing and reducing conditions, respectively. A working concentration of the capture antibody at 1-2 µ g/ml (100µl/well) in combination with a biotinylated polyclonal detection antibody at 25-50 ng/ml (100µl/well) is recommended to detect human interleukin 7.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
Sequence similaritiesBelongs to the IL-7/IL-9 family.