Overview

  • Product name
  • Description
    Rabbit polyclonal to IL7
  • Tested applications
    Suitable for: WB, ELISA, Neutralising, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mIL-7 (mouse Interleukin-7)

  • Positive control
    • IHC-P: FFPE mouse spleen tissue sections.

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-7 (using 100 µl/well antibody solution).
Neutralising Use a concentration of 0.08 - 0.1 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mIL-7 (0.50 ng/ml).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
  • Sequence similarities
    Belongs to the IL-7/IL-9 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IL 7 antibody
    • IL-7 antibody
    • Il7 antibody
    • IL7_HUMAN antibody
    • Interleukin 7 antibody
    • Interleukin-7 antibody
    • LP-1 antibody
    • Lymphopoietin 1 antibody
    see all

Anti-IL7 antibody images

  • IHC image of IL7 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9732, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-IL7 antibody (ab9732)

This product has been referenced in:
  • Wei J  et al. Lamotrigine decreases MRP8 and IL-7 in rat models of intractable epilepsy secondary to focal cortical dysplasia. Exp Ther Med 12:3694-3698 (2016). Read more (PubMed: 28105099) »
  • Parrish YK  et al. IL-7 Dependence in human B lymphopoiesis increases during progression of ontogeny from cord blood to bone marrow. J Immunol 182:4255-66 (2009). Neutralising ; Mouse . Read more (PubMed: 19299724) »

See all 2 Publications for this product

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There are currently no Abreviews or Questions for ab9732.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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