Overview

  • Product name
    Anti-IL7 antibody (Biotin)
    See all IL7 primary antibodies
  • Description
    Rabbit polyclonal to IL7 (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IL7 aa 26-177. Immunogen is the mature full length protein without the signal peptide from aa 1-25
    Sequence:

    MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH


    Database link: P13232

  • General notes
    A sterile filtered antibody solution was lyophilized

Properties

  • Form
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa.

To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA Use a concentration of 0.25 - 1 µg/ml.

To detect Human IL-7 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. ab84254, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IL-7.

Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL7 (ab9628).

To detect Human IL-7 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. ab84254, in conjunction with ab9628 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IL-7.

Target

  • Function
    Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
  • Sequence similarities
    Belongs to the IL-7/IL-9 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IL 7 antibody
    • IL-7 antibody
    • Il7 antibody
    • IL7_HUMAN antibody
    • Interleukin 7 antibody
    • Interleukin-7 antibody
    • LP-1 antibody
    • Lymphopoietin 1 antibody
    see all

References for Anti-IL7 antibody (Biotin) (ab84254)

ab84254 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab84254.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up