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Recombinant full length protein corresponding to Human IL7 aa 26-177. Immunogen is the mature full length protein without the signal peptide from aa 1-25
MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH
Our Abpromise guarantee covers the use of ab84254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa.
To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL7 (ab9628).
To detect Human IL-7 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. ab84254, in conjunction with ab9628 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IL-7.
ab84254 has not yet been referenced specifically in any publications.