Anti-IL7R alpha (phospho Y449) antibody (ab73341)

Overview

  • Product nameAnti-IL7R alpha (phospho Y449) antibody
    See all IL7R alpha primary antibodies
  • Description
    Rabbit polyclonal to IL7R alpha (phospho Y449)
  • Specificityab73341 detects endogenous levels of IL7R alpha only when phosphorylated at tyrosine 449.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human IL7R alpha around the phosphorylation site of tyrosine 449 (EAYPVT)

  • Positive control
    • HeLa cell extract. HUVEC cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab73341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionReceptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
  • Involvement in diseaseDefects in IL7R are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in IL7R are a cause of susceptibility to multiple sclerosis type 3 (MS3) [MIM:612595]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=A polymorphism at position 244 strongly influences susceptibility to multiple sclerosis. Overtransmission of the major 'C' allele coding for Thr-244 is detected in offspring affected with multiple sclerosis. In vitro analysis of transcripts from minigenes containing either 'C' allele (Thr-244) or 'T' allele (Ile-244) shows that the 'C' allele results in an approximately two-fold increase in the skipping of exon 6, leading to increased production of a soluble form of IL7R. Thus, the multiple sclerosis associated 'C' risk allele of IL7R would probably decrease membrane-bound expression of IL7R. As this risk allele is common in the general population, some additional triggers are probably required for the development and progression of MS.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications
    N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 127 antibody
    • CD127 antibody
    • CD127 antigen antibody
    • CDw127 antibody
    • IL 7R alpha antibody
    • IL 7R antibody
    • IL-7 receptor subunit alpha antibody
    • IL-7R subunit alpha antibody
    • IL-7R-alpha antibody
    • IL-7RA antibody
    • IL7R antibody
    • IL7RA antibody
    • IL7RA_HUMAN antibody
    • IL7Ralpha antibody
    • ILRA antibody
    • Interleukin 7 receptor alpha chain antibody
    • Interleukin 7 receptor antibody
    • Interleukin 7 receptor isoform H5 6 antibody
    • Interleukin-7 receptor subunit alpha antibody
    see all

Anti-IL7R alpha (phospho Y449) antibody images

  • All lanes : Anti-IL7R alpha (phospho Y449) antibody (ab73341) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of IL7R alpha in HUVEC cells using ab73341 at 1/500 dilution in the presence (right panel) or absence (left panel) of immunising phosphopeptide.

References for Anti-IL7R alpha (phospho Y449) antibody (ab73341)

ab73341 has not yet been referenced specifically in any publications.

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